{"artifact":{"id":"paper-fig-paper-41692246-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5 — Lipidome and proteome of astrocyte and microglia ApoE lipoprotein reveal differe","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-41692246\", \"timestamp\": \"2026-04-22T01:29:42.053447\"}]","content_hash":null,"metadata":{"pmid":"paper-41692246","caption":"A: Volcano plot showing differential protein abundance of proteins bound to astrocyte versus microglia immunopurified lipoprotein. Proteins involved in glucose metabolism (Pkm, HECT domain E3 ubiquitin protein ligase 4 [Hectd4], and ApoD) and acute phase response (fibronectin 1 [Fn1] and Serpina3n) are differentially abundant in astrocyte ApoE lipoproteins. Proteins involved in complement activation (C1qa, C1qb, and complement factor I [CfI]), synapse pruning (C1qa and C1qb), proteolysis (matrix metallopeptidase 12 [Mmp12], serine carboxypeptidase 2 [Cpb2], CfI, and cathepsin B [Ctsb]), and the innate immune response (C1qa, C1qb, and CfI) are differentially abundant in microglia ApoE lipoprotein. For proteomics analysis, the following groups were analyzed: ApoE2 astrocytes (n = 5), ApoE3 astrocytes (n = 5), ApoE4 astrocytes (n = 5), ApoE2 microglia (n = 3), ApoE3 microglia (n = 3), and ApoE4 microglia (n = 4). B: PCA of the proteome of astrocyte and microglia ApoE lipoproteins. Circles","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12996666/bin/gr5.jpg","paper_title":"Lipidome and proteome of astrocyte and microglia ApoE lipoprotein reveal differences based on cell type and ApoE isoform.","figure_label":"Fig. 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"6f62c109-3444-4424-a84a-183bb516d3cd","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-41692246-5","is_canonical":true,"supersede_chain":["paper-fig-paper-41692246-5"]}