{"artifact":{"id":"paper-fig-paper-5839e7d0e39b-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 2 — Small nucleoli are a cellular hallmark of longevity.","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-5839e7d0e39b\", \"timestamp\": \"2026-04-22T01:29:42.540891\"}]","content_hash":null,"metadata":{"pmid":"paper-5839e7d0e39b","caption":"Nucleolar size inversely correlates with longevity. ( a )  eat-2(ad465)  animals have smaller nucleoli while  ncl-1(e1942)  and  eat-2;ncl-1  animals possess larger nucleoli compared to N2. (20 worms imaged per replicate, 3 independent biological replicates) ( b ) Nucleolar size is reduced on bacterial food reduction with a corresponding increase in lifespan. ( P <0.0001, log-rank test). ( c , d )  eat-2(ad465) , TOR RNAi,  isp-1(qm150) ,  glp-1(e2141)  and  daf-2(e1370)  animals possess smaller nucleoli while  daf-2;daf-16  have nucleoli similar to N2 in the hypodermis and pharyngeal muscle (Error bars represent mean±s.d.) ( e , f ) Schematic illustrating the experiment, which shows that longer-lived worms exhibit small nucleoli and vice versa. (The graph depicts mean and s.d. Pearson correlation coefficient  R 2 =0.93 is calculated using the entire data set, the line equation is  y =−0.1483x+6.9148). Scale bar represents 5 μm. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001, NS, ","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC5582349/bin/ncomms16083-f2.jpg","paper_title":"Small nucleoli are a cellular hallmark of longevity.","figure_label":"Figure 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"39767936-c5ef-4f21-b1c2-ffcc61920c8f","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-5839e7d0e39b-2","is_canonical":true,"supersede_chain":["paper-fig-paper-5839e7d0e39b-2"]}