{"artifact":{"id":"paper-fig-paper-5839e7d0e39b-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 3 — Small nucleoli are a cellular hallmark of longevity.","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-5839e7d0e39b\", \"timestamp\": \"2026-04-22T01:29:42.541750\"}]","content_hash":null,"metadata":{"pmid":"paper-5839e7d0e39b","caption":"Longevity mutants have reduced ribosome biogenesis. ( a ) FIB-1::GFP is strongly down-regulated in  eat-2(ad465)  animals but up-regulated in  ncl-1(e1942)  and  eat-2;ncl-1  double mutants (20 worms imaged per replicate, 3 independent biological replicates). ( b – d ) rRNA, RPS6, RPS15 and FIB-1 levels are increased in  ncl-1(e1942)  and reduced in  eat-2(ad465) ,  isp-1(qm150) ,  glp-1(e2141) ,  daf-2  RNAi and TOR RNAi and this effect is partially reversed by the loss of  ncl-1. daf-2(e1370)  also shows reduced rRNA levels compared to  daf-2;daf-16(mu86)  (Error bars represent mean±s.e.m.). ( e )  fib-1  RNAi extends lifespan of N2 ( P =0.0004, log-rank test, three independent biological replicates) ( f )  fib-1  RNAi reduces the nucleolar size of N2 (Error bars represent mean±s.d.). Scale bar represents 20 μm. * P <0.05, ** P <0.01, **** P <0.0001, unpaired  t -test.","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC5582349/bin/ncomms16083-f3.jpg","paper_title":"Small nucleoli are a cellular hallmark of longevity.","figure_label":"Figure 3","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"7a7ad050-a8b5-41f5-afae-88681eed4e45","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-5839e7d0e39b-3","is_canonical":true,"supersede_chain":["paper-fig-paper-5839e7d0e39b-3"]}