{"artifact":{"id":"paper-fig-paper-5fa244f809e0-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 4. — MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers.","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-5fa244f809e0\", \"timestamp\": \"2026-04-22T01:29:42.746492\"}]","content_hash":null,"metadata":{"pmid":"paper-5fa244f809e0","caption":"Reconstitution of miR223-3p induces synthetic lethality in HR-deficient cancers. ( A ) QRT-PCR comparing the levels of miR223-3p and miR223-5p in Jurkat cells (HR-replete), MDA-MB-436 (BRCA1-mutant breast cancer cell line), and UWB1.289 (BRCA1-mutant ovarian cancer cell line). ( B ) QRT-PCR showing the expression of miR223-3p following transfection with miR223-3p in MDA-MB-436 breast cancer cells. ( C ) Clonogenic survival assay showing the number of colonies in MDA-MB-435, UWB1.289, and MCF7 (HR-replete breast cancer) cells upon transfection of miR223-3p. ( D ) Clonogenic survival assay in MDA-MB-436 after depletion of PARP1, CtIP, and PSO4. ( E ) Comparison of number of colonies in MDA-MB-436, and HCC-1937 (BRCA1-mutant breast cancer) cells in the presence of olaparib. ( F ) Clonogenic survival assay in HCC1937 cells after miR223-3p transfection.","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC6717293/bin/pnas.1903150116fig04.jpg","paper_title":"MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers.","figure_label":"Fig. 4.","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"5023b56c-01ef-42f9-8b28-99fab7dab463","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-5fa244f809e0-4","is_canonical":true,"supersede_chain":["paper-fig-paper-5fa244f809e0-4"]}