{"artifact":{"id":"paper-fig-paper-60585853d1d9-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 2 — HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regula","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-60585853d1d9\", \"timestamp\": \"2026-04-22T01:29:42.827115\"}]","content_hash":null,"metadata":{"pmid":"paper-60585853d1d9","caption":"The cytosolic DNA sensor cGAS is activated after cerebral ischemia/reperfusion.  ( A ) The mRNA level of cGAS in primary microglia isolated from brain tissue 3 h post-tMCAO (9 mice per group). ( B ) The protein level of cGAS in primary microglia isolated from brain tissue at 1, 2, 3 and 6 h post-tMCAO, respectively. The gray values were analyzed by using ImageJ and were normalized to Iba1. ( C ) BV2 cells were transfected with siRNA against cGAS and harvested 48 h post-transfection to quantify the mRNA level of cGAS. ( D ) cGAS-silenced and control BV2 cells were transfected with poly(dA:dT) and harvested 3 h post-poly(dA:dT) treatment to analyze the protein levels of pIRF3, cGAS, and GAPDH. ( E ) cGAS-silenced, STING-silenced, and control BV2 cells were transfected with poly(dA:dT) and harvested 6 h post-poly(dA:dT) treatment to analyze the mRNA level of IFN-β. ( F ) BV2 cells were transfected with siRNA against STING and harvested 48 h post-transfection to quantify the mRNA level of ","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC7449914/bin/thnov10p9644g002.jpg","paper_title":"HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regulating the microglial cGAS-STING pathway.","figure_label":"Figure 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"4d8ccc1c-899b-404e-b192-100f5d43bec3","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-60585853d1d9-2","is_canonical":true,"supersede_chain":["paper-fig-paper-60585853d1d9-2"]}