{"artifact":{"id":"paper-fig-paper-60585853d1d9-6","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 6 — HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regula","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-60585853d1d9\", \"timestamp\": \"2026-04-22T01:29:42.830382\"}]","content_hash":null,"metadata":{"pmid":"paper-60585853d1d9","caption":"Deficiency of HDAC3 in microglia suppresses the cGAS-STING-mediated autoimmune response . ( A  and  B ) Adult primary microglial cells were isolated from HDAC3 cKO (n = 6) and WT (n = 6) mice, and the mRNA levels of HDAC3 (A) and cGAS (B) were analyzed by real-time PCR. ( C ) The neurological deficits of HDAC3 cKO (n = 7) and WT (n = 15) mice subjected to tMCAO were assessed 24 h post-reperfusion. ( D  and  E ) Brain tissue isolated from WT (n = 14) and HDAC3 cKO (n = 7) mice 24 h post-reperfusion was stained with triphenyl tetrazolium chloride, and infarct volume was determined using ImageJ (D) and infarct volume was determined using Image J (E). ( F ) RNA was extracted from the ischemic penumbra cortex and contra cortex of HDAC3 cKO (n = 12) and WT (n = 14) mice subjected to tMCAO 6 h post-reperfusion, and the mRNA level of IL-6 was quantified by real-time PCR. ( G ) Serum was isolated from HDAC3 cKO (n = 13) and WT (n = 21) mice subjected to tMCAO 6 h post-reperfusion, and the level","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC7449914/bin/thnov10p9644g006.jpg","paper_title":"HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regulating the microglial cGAS-STING pathway.","figure_label":"Figure 6","figure_number":6,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"a4577e30-0978-4d9e-91df-35d84f59065a","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-60585853d1d9-6","is_canonical":true,"supersede_chain":["paper-fig-paper-60585853d1d9-6"]}