{"artifact":{"id":"paper-fig-paper-8281d7c4ac82-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5 — A novel derivative of valepotriate inhibits the PI3K/AKT pathway and causes Noxa","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-8281d7c4ac82\", \"timestamp\": \"2026-04-22T01:29:42.776362\"}]","content_hash":null,"metadata":{"pmid":"paper-8281d7c4ac82","caption":"Amcp disrupted the mitochondrial membrane potential and affected mitochondrial proteins. a  BxPC-3 and SW1990 cells were seeded into six-well plates at a density of 2 × 10 5 /well and were cultured for 24 h. After treatment with Amcp for 12 h, JC-1 staining was used to observe the depolarized mitochondrial membrane potential. Cells were analyzed by flow cytometry.  b  Quantitative analysis of cells with a depolarized mitochondria membrane.  c  Cells were treated with the indicated concentrations of Amcp or gemcitabine (Gem) for 24 h before Western blotting assays. * P  < 0.05.  d  The protein level of Noxa was dramatically reduced by Noxa siRNA knockdown in BxPC-3 cells.  e  BxPC-3 cells were exposed to Amcp for 24 h with or without Noxa knockdown. Cell viability was determined by CCK-8 assays.  f  Noxa was knocked down by siRNA in BxPC-3 cells before treatment and cells were exposed to the indicated concentrations of Amcp or gemcitabine for 24 h before Western blotting analysis.  g  B","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC7470838/bin/41401_2019_354_Fig5_HTML.jpg","paper_title":"A novel derivative of valepotriate inhibits the PI3K/AKT pathway and causes Noxa-dependent apoptosis in human pancreatic cancer cells.","figure_label":"Fig. 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"4fac9781-c2b1-4241-b16b-01a9c218f228","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-8281d7c4ac82-5","is_canonical":true,"supersede_chain":["paper-fig-paper-8281d7c4ac82-5"]}