{"artifact":{"id":"paper-fig-paper-828b7c4fa9a6-1","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 1 — C1q propagates microglial activation and neurodegeneration in the visual axis fo","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-828b7c4fa9a6\", \"timestamp\": \"2026-04-22T01:29:42.451109\"}]","content_hash":null,"metadata":{"pmid":"paper-828b7c4fa9a6","caption":"Retinal I/R injury significantly increases C1q expression in the retina. C57BL/6 J mice were subjected to unilateral I/R injury and sacrificed at the indicated time points post I/R. Contralateral control eyes ( a ) displayed basal deposits of C1q in the GCL; however, ischemic eyes ( b – f ) had significantly greater expression of C1q as early as day 3 ( b ) and sustained to the end of the time course on day 28 ( f ). Positive labeling was only observed in the GCL and IPL. ( g ) Fluorescence intensity quantification revealed statistically significant upregulation of C1q ( p  < 0.01) at all time points in ischemic versus uninjured retinas. Peak intensity in ischemic eyes was observed on day 21 post injury ( p  < 0.05). Mean ± SEM,  n  = 7 per group. ** p  < 0.01 determined by student’s paired  t -test, # p  < 0.05 tested using One-way ANOVA followed by Holm-Sidak post test. GCL = Ganglion Cell Layer, INL = Inner Nuclear Layer, ONL = Outer Nuclear Layer. Scale bars = 50 μm","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC4806521/bin/13024_2016_89_Fig1_HTML.jpg","paper_title":"C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury.","figure_label":"Fig. 1","figure_number":1,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"e32b7866-f1c8-476d-ba30-632881730d2e","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-828b7c4fa9a6-1","is_canonical":true,"supersede_chain":["paper-fig-paper-828b7c4fa9a6-1"]}