{"artifact":{"id":"paper-fig-paper-828b7c4fa9a6-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 3 — C1q propagates microglial activation and neurodegeneration in the visual axis fo","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-828b7c4fa9a6\", \"timestamp\": \"2026-04-22T01:29:42.453171\"}]","content_hash":null,"metadata":{"pmid":"paper-828b7c4fa9a6","caption":"Activation and increased cell populations of macroglia and microglia in the retina post I/R. Representative images of Iba1- and GFAP-positive cells in uninjured ( a ), ( c ) and ischemic ( b ), ( d ) retinas.  a ,  b  Yellow inset boxes of selected Iba1 +  microglia demonstrate morphological differences between senescent (i) and activated (ii) cellular states.  c ,  d  Morphological differences observed in GFAP +  cells following ischemia. Astrocytes and Müller cells display elongated processes extending through the layers of the retina in addition to thickened cell bodies present in the GCL and NFL.  e ,  f  Thresholding analysis based upon percent area of retina occupied was applied to determine changes in cell density. Microglial density ( e ) was significantly increased in ischemic retinas at all time points quantified compared with uninjured controls. Density of macroglia ( f ) trended upward on day 3 and was statistically significant at all time points measured thereafter. Mean ±","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC4806521/bin/13024_2016_89_Fig3_HTML.jpg","paper_title":"C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury.","figure_label":"Fig. 3","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"f0e92009-7a37-43e7-a6ee-3ad2480d243e","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-828b7c4fa9a6-3","is_canonical":true,"supersede_chain":["paper-fig-paper-828b7c4fa9a6-3"]}