{"artifact":{"id":"paper-fig-paper-862a1a9ceb7a-15","artifact_type":"paper_figure","entity_ids":"[]","title":"Extended Data Fig. 10 — B cells orchestrate tolerance to the neuromyelitis optica autoantigen AQP4.","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-862a1a9ceb7a\", \"timestamp\": \"2026-04-22T01:29:41.556326\"}]","content_hash":null,"metadata":{"pmid":"paper-862a1a9ceb7a","caption":"AQP4-directed autoimmunity in mice recapitulates histological hallmarks of NMOSD. ( a ) Representative CD45, AQP4, and GFAP stainings of the spinal cord, cerebellum, and ependyma of P41-immunized  Aqp4 ΔB  mice at the peak of EAE (scale bars top row 1 mm except for spinal cord 200 µm, all other rows 100 µm). ( b ) AQP4 loss was determined in a semi-quantitative approach normalized to the extent of adjacent CNS lesions defined by CD45 +  immunoreactivity. The AQP4 signal was calculated by QuPath’s positive pixel count algorithm in the adjacent area with a defined radius of 100 µm with respect to CD45 +  infiltrates (region of interest). Data are shown as mean ± SD and tested with a two-tailed unpaired t-test. * P < 0.05, ** P < 0.01, *** P < 0.001. Symbols represent individual CNS lesions detected in two biological replicates for each group. \n Source Data","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC10937377/bin/41586_2024_7079_Fig15_ESM.jpg","paper_title":"B cells orchestrate tolerance to the neuromyelitis optica autoantigen AQP4.","figure_label":"Extended Data Fig. 10","figure_number":15,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"6f553539-b96a-4ac0-96ae-3dbe62b51f67","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-862a1a9ceb7a-15","is_canonical":true,"supersede_chain":["paper-fig-paper-862a1a9ceb7a-15"]}