{"artifact":{"id":"paper-fig-paper-de0f65fde5fe-5","artifact_type":"paper_figure","entity_ids":"[]","title":"FIGURE 5 — The extracellular matrix component perlecan/HSPG2 regulates radioresistance in p","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-de0f65fde5fe\", \"timestamp\": \"2026-04-22T01:29:41.536890\"}]","content_hash":null,"metadata":{"pmid":"paper-de0f65fde5fe","caption":"Validation of the finding using androgen-responsive LNCaP parental (P) and radioresistant (RR) cells.  (A)  Analysis of the relative cell radiosensitivity of LNCaP radioresistant (RR) and parental (P) cells using radiobiological colony formation assay. Data are mean ± SD; *** p  < 0.001.  (B)  The cell adhesion assay reveals that LNCaP P cells show enhanced adhesion properties to their own, cell derived matrix than LNCaP RR cells. Data are mean ± SD; * p  < 0.05.  (C)  Western blot analysis of ECM protein COL4A2 in LNCaP P and LNCaP RR cells. Forty-eight hours after seeding, integrin nexus proteins were isolated, and WB analysis was performed.  (D)  RT-qPCR analysis of the relative perlecan/HSPG2 expression in LNCaP P and RR cells. Cells were transfected with perlecan/HSPG2 siRNA or scrambled (Scr) siRNA as a control. N = 3; Error bars = SD; ** p  < 0.01; *** p  < 0.001.  (E)  RNA seq analysis of the relative perlecan/HSPG2 expression in LNCaP P cells. Cells were transfected with AR si","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11325029/bin/fcell-12-1452463-g005.jpg","paper_title":"The extracellular matrix component perlecan/HSPG2 regulates radioresistance in prostate cancer cells.","figure_label":"FIGURE 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"47ea4a28-5cbb-4946-b3ac-40e3f9ba6bac","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-de0f65fde5fe-5","is_canonical":true,"supersede_chain":["paper-fig-paper-de0f65fde5fe-5"]}