{"artifact":{"id":"paper-fig-paper-e86f071aad74-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Extended Data Fig. 3. — Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with gl","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-e86f071aad74\", \"timestamp\": \"2026-04-22T01:29:41.388669\"}]","content_hash":null,"metadata":{"pmid":"paper-e86f071aad74","caption":"MTKO mice are susceptible to obesity and have reduced energy expenditure on a high-fat diet. a,  Mice were fed a high-fat diet (HFD) beginning at 15 weeks of age, and the percentage weight gain from baseline is plotted. N=10 WT and 12 MTKO mice.  b,  After the HFD, HOMA-IR was calculated from paired measurements of plasma glucose and insulin. N=7 WT and 10 MTKO mice.  c,  Immunoblots were done as indicated on hindlimb muscles from mice fed a HFD for 3 weeks.  d–f,  Body weights and composition of 14-week old MTKO and WT mice fed a HFD for 3 weeks prior to measurements done in metabolic cages. N=7 in each group.  g–q,  HFD-fed 14-week old MTKO and WT mice were housed in metabolic cages and the indicated parameters were measured. N=7 in each group (g–l, p, q); N=7 WT and 6 MTKO mice (m–o). RER, respiratory exchange ratio; LBM, lean body mass. All data are presented as mean ± SEM of biologically independent samples, analyzed using two-tailed t-tests. *p<0.05, **p<0.01, ***p<0.001.","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC7990718/bin/nihms-1671263-f0010.jpg","paper_title":"Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake.","figure_label":"Extended Data Fig. 3.","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"1851de3a-6f49-4894-a0ce-7fed619ae9a0","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-e86f071aad74-3","is_canonical":true,"supersede_chain":["paper-fig-paper-e86f071aad74-3"]}