{"artifact":{"id":"rsc-h-SDA-2026-04-26-gap-6734250e","artifact_type":"rigor_score_card","entity_ids":null,"title":"Rigor Score Card: Dominant-Negative Spliceosome Titration","quality_score":0.225,"created_by":"rigor_score_card","provenance_chain":"[{\"artifact_id\": \"h-SDA-2026-04-26-gap-pubmed-20260412-094853-199f4f1b-01-dominant-negative-spliceosome-titration-20b316433d\", \"relation\": \"scores\"}]","content_hash":null,"metadata":{"dimensions":["scientific_premise","study_design","blinding","power_analysis","resource_identification","statistical_reporting","data_availability","sabv"],"eval_a_raw":{"scores":{"sabv":{"score":1,"evidence":"Dominant-Negative Spliceosome Titration","location":"no mention of sex considerations or disaggregated analysis"},"blinding":{"score":1,"evidence":"The mechanism explains why a minority aberrant isoform disproportionately affects protein output beyond simple haploinsufficiency.","location":"no mention of assessors, analysts, or blinding procedures"},"study_design":{"score":1,"evidence":"This cis-trans interference causes inefficient removal of downstream introns, producing additional aberrant transcripts with PTCs that are degraded by NMD, establishing a positive feedback loop that progressively depletes mature GBA mRNA and protein.","location":"single paragraph - no experimental design described"},"power_analysis":{"score":1,"evidence":"establishing a positive feedback loop that progressively depletes mature GBA mRNA and protein.","location":"no power analysis, sample size justification, or statistical planning mentioned"},"data_availability":{"score":1,"evidence":"The mechanism explains why a minority aberrant isoform disproportionately affects protein output beyond simple haploinsufficiency.","location":"no data sharing, code repository, or accessibility statement"},"scientific_premise":{"score":1,"evidence":"Intron-retained GBA transcripts sequester core spliceosomal components (U2AF65, SF3B1, PRPF8) and snRNPs, reducing the available pool for wild-type GBA pre-mRNA processing.","location":"single paragraph hypothesis statement"},"statistical_reporting":{"score":1,"evidence":"producing additional aberrant transcripts with PTCs that are degraded by NMD","location":"no statistical tests, thresholds, corrections, or assumptions reported"},"resource_identification":{"score":1,"evidence":"U2AF65, SF3B1, PRPF8","location":"proteins mentioned without RRID, catalog numbers, or source verification"}},"overall_summary":"This submission is a mechanistic hypothesis statement entirely lacking methodological rigor—no study design, statistical methods, power analysis, blinding, resource identification with RRIDs, data availability statements, or sex consideration are present. The text proposes a speculative mechanism without citation of prior work, knowledge gap articulation, or any experimental grounding.","weakest_dimension":"study_design","strongest_dimension":"scientific_premise"},"eval_b_raw":{"scores":{"sabv":{"score":1,"evidence":"No mention of sex as a biological variable, no disaggregation by sex, no consideration of sex-specific effects on the GBA mechanism.","location":"Overall - sex not considered"},"blinding":{"score":1,"evidence":"No mention of blinding, assessor independence, or allocation concealment. Not applicable to this hypothesis text.","location":"Overall - no blinding references"},"study_design":{"score":1,"evidence":"This is a hypothesis/mechanistic proposal. No experimental design, cohort, intervention, or observational study is described. All verbs are assertive/causative rather than reporting methods.","location":"Overall - no study design section exists"},"power_analysis":{"score":1,"evidence":"No statistical power analysis, sample size justification, or effect size estimation is provided. No N values or confidence intervals mentioned.","location":"Overall - no power calculations"},"data_availability":{"score":1,"evidence":"No data deposition statements, no code repository links, no raw data availability, no supplemental material references.","location":"Overall - no data sharing"},"scientific_premise":{"score":2,"evidence":"Intron-retained GBA transcripts sequester core spliceosomal components (U2AF65, SF3B1, PRPF8) and snRNPs","location":"Hypothesis statement, sentence 1"},"statistical_reporting":{"score":1,"evidence":"No statistical tests, P-values, alpha thresholds, multiple comparison corrections, or distributional assumptions reported. Purely mechanistic assertion.","location":"Overall - no statistical methods"},"resource_identification":{"score":1,"evidence":"No RRIDs for reagents, no catalog numbers for antibodies (U2AF65, SF3B1, PRPF8), no cell line authentication, no dataset accession numbers.","location":"Overall - no resource identifiers"}},"overall_summary":"This text is a mechanistic hypothesis statement rather than a report of empirical findings. It proposes a dominant-negative spliceosome titration model but lacks any methodological rigor: no study design, no statistics, no resources identified, no data availability, and no SABV consideration. The scientific premise is minimally acceptable (specific molecular players named) but lacks citation of prior literature establishing this mechanism.","weakest_dimension":"study_design - this is not a study at all; it's an untested hypothesis with no experimental or observational framework described","strongest_dimension":"scientific_premise - the text includes specific molecular components (U2AF65, SF3B1, PRPF8, snRNPs) and proposes a mechanistically detailed model, though still lacking citations"},"provider_a":"minimax","provider_b":"glm","reconciled":{"reconciler_notes":"High inter-rater agreement observed across all 8 dimensions. For scientific_premise, Evaluator A gave a score of 1 while Evaluator B gave a 2 (difference = 1, within threshold); the reconciled score is 2 (average of 1 and 2 rounded). All other dimensions show perfect agreement at score 1. The reconciled scores reflect that this text is a mechanistic hypothesis statement lacking experimental design, statistical methods, resource identifiers, data availability, blinding considerations, power analysis, and sex-as-a-biological-variable consideration. The only marginally acceptable element is the scientific premise, which names specific molecular components (U2AF65, SF3B1, PRPF8, snRNPs) but still lacks citations to prior work.","overall_agreement":"high","reconciled_scores":{"sabv":{"score":1,"a_score":1,"b_score":1,"a_evidence":"Dominant-Negative Spliceosome Titration","b_evidence":"No mention of sex as a biological variable, no disaggregation by sex, no consideration of sex-specific effects on the GBA mechanism.","disagreement":false},"blinding":{"score":1,"a_score":1,"b_score":1,"a_evidence":"The mechanism explains why a minority aberrant isoform disproportionately affects protein output beyond simple haploinsufficiency.","b_evidence":"No mention of blinding, assessor independence, or allocation concealment. Not applicable to this hypothesis text.","disagreement":false},"study_design":{"score":1,"a_score":1,"b_score":1,"a_evidence":"This cis-trans interference causes inefficient removal of downstream introns, producing additional aberrant transcripts with PTCs that are degraded by NMD, establishing a positive feedback loop that progressively depletes mature GBA mRNA and protein.","b_evidence":"This is a hypothesis/mechanistic proposal. No experimental design, cohort, intervention, or observational study is described. All verbs are assertive/causative rather than reporting methods.","disagreement":false},"power_analysis":{"score":1,"a_score":1,"b_score":1,"a_evidence":"establishing a positive feedback loop that progressively depletes mature GBA mRNA and protein.","b_evidence":"No statistical power analysis, sample size justification, or effect size estimation is provided. No N values or confidence intervals mentioned.","disagreement":false},"data_availability":{"score":1,"a_score":1,"b_score":1,"a_evidence":"The mechanism explains why a minority aberrant isoform disproportionately affects protein output beyond simple haploinsufficiency.","b_evidence":"No data deposition statements, no code repository links, no raw data availability, no supplemental material references.","disagreement":false},"scientific_premise":{"score":2,"a_score":1,"b_score":2,"a_evidence":"Intron-retained GBA transcripts sequester core spliceosomal components (U2AF65, SF3B1, PRPF8) and snRNPs, reducing the available pool for wild-type GBA pre-mRNA processing.","b_evidence":"Intron-retained GBA transcripts sequester core spliceosomal components (U2AF65, SF3B1, PRPF8) and snRNPs","disagreement":false},"statistical_reporting":{"score":1,"a_score":1,"b_score":1,"a_evidence":"producing additional aberrant transcripts with PTCs that are degraded by NMD","b_evidence":"No statistical tests, P-values, alpha thresholds, multiple comparison corrections, or distributional assumptions reported. Purely mechanistic assertion.","disagreement":false},"resource_identification":{"score":1,"a_score":1,"b_score":1,"a_evidence":"U2AF65, SF3B1, PRPF8","b_evidence":"No RRIDs for reagents, no catalog numbers for antibodies (U2AF65, SF3B1, PRPF8), no cell line authentication, no dataset accession numbers.","disagreement":false}},"inter_rater_agreement":"1.0","dimensions_with_disagreement":[]},"_schema_version":1,"scored_entity_id":"h-SDA-2026-04-26-gap-pubmed-20260412-094853-199f4f1b-01-dominant-negative-spliceosome-titration-20b316433d","scored_entity_type":"hypothesis","scored_entity_title":"Dominant-Negative Spliceosome Titration"},"created_at":"2026-04-27T01:15:27.106650-07:00","updated_at":"2026-04-27T01:15:27.106655-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"internal","origin_url":null,"lifecycle_changed_at":null,"citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"0d4fa342-420c-4806-b0c2-7b5899c67334","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"rigor_score_card"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"rsc-h-SDA-2026-04-26-gap-6734250e","is_canonical":true,"supersede_chain":["rsc-h-SDA-2026-04-26-gap-6734250e"]}