Semantic Diff — protein_design-04aa5832-ce91-4020-9aa9-7093f481ccf2 v4→v5
Summary: score 0.720→0.820 (+0.100); 4 fields changed; 7 metadata keys changed
Changes
| Field | Before | After | Interpretation |
|---|---|---|---|
| title | TREM2 Ectodomain Variant — AlphaFold Base | TREM2 Ectodomain Variant — Stability Optimized | |
| quality_score | 0.72 | 0.82 | |
| version_tag | initial | stability-opt | |
| changelog | None | Introduced 3 stabilizing mutations from Rosetta energy minimization | |
| metadata.binding_affinity | {'kd_nm': 850, 'target': 'Aβ oligomers'} | {'kd_nm': 780, 'target': 'Aβ oligomers'} | |
| metadata.delta_G_kcal | None | -2.3 | |
| metadata.design_rationale | Wild-type ectodomain structure as baseline for optimization | Rosetta-guided mutations to improve thermostability without disrupting binding interface | |
| metadata.method | alphafold | rosetta_relax | |
| metadata.mutations | [] | ['T96S', 'K186R', 'A192V'] | |
| metadata.plddt_score | 0.89 | None | |
| metadata.predicted_stability | 0.72 | 0.91 |
Text Diff
--- v2
+++ v3
@@ -1,12 +1,13 @@
-title: TREM2 Ectodomain Variant — AlphaFold Base
-quality_score: 0.72
+title: TREM2 Ectodomain Variant — Stability Optimized
+quality_score: 0.82
lifecycle_state: active
-version_tag: initial
-metadata.binding_affinity: {'kd_nm': 850, 'target': 'Aβ oligomers'}
-metadata.design_rationale: Wild-type ectodomain structure as baseline for optimization
-metadata.method: alphafold
-metadata.mutations: []
-metadata.plddt_score: 0.89
-metadata.predicted_stability: 0.72
+version_tag: stability-opt
+changelog: Introduced 3 stabilizing mutations from Rosetta energy minimization
+metadata.binding_affinity: {'kd_nm': 780, 'target': 'Aβ oligomers'}
+metadata.delta_G_kcal: -2.3
+metadata.design_rationale: Rosetta-guided mutations to improve thermostability without disrupting binding interface
+metadata.method: rosetta_relax
+metadata.mutations: ['T96S', 'K186R', 'A192V']
+metadata.predicted_stability: 0.91
metadata.sequence: MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG
metadata.uniprot_id: Q9NZC2