Semantic Diff — protein_design-2de03617-e4e6-4d54-9fdb-8556ea00ac80 v5→v6
Summary: score 0.820→0.880 (+0.060); 4 fields changed; 8 metadata keys changed
Changes
| Field | Before | After | Interpretation |
|---|---|---|---|
| title | TREM2 Ectodomain Variant — Stability Optimized | TREM2 Ectodomain Variant — Binding Optimized | |
| quality_score | 0.82 | 0.88 | |
| version_tag | stability-opt | binding-opt | |
| changelog | Introduced 3 stabilizing mutations from Rosetta energy minimization | Interface mutations to enhance Aβ binding while maintaining stability gains | |
| metadata.binding_affinity | {'kd_nm': 780, 'target': 'Aβ oligomers'} | {'kd_nm': 120, 'target': 'Aβ oligomers'} | |
| metadata.delta_G_kcal | -2.3 | -1.8 | |
| metadata.design_rationale | Rosetta-guided mutations to improve thermostability without disrupting binding interface | Interface residue optimization for enhanced Aβ oligomer recognition. Slight stability trade-off accepted for 6.5x binding improvement. | |
| metadata.method | rosetta_relax | rosetta_interface_design | |
| metadata.mutations | ['T96S', 'K186R', 'A192V'] | ['T96S', 'K186R', 'A192V', 'H157Y', 'R62W'] | |
| metadata.plddt_score | 0.92 | 0.9 | |
| metadata.predicted_stability | 0.91 | 0.88 | |
| metadata.sequence | MEPLRPPWHLLLLCLCLLSLLPSGFSTEETVSTGPLEKRQLLTRGNIFSQTENLRQGEQDSFPIYVYSLGLGGSPSVQCNVRQGLSWTHEFTVTYTDLVPNGGSCSNFSSMRYHRSRRCSVRVYTLSITGITPSLRYHMKQQGKLLVTLPQQAVQDFPLWLQVNFSDKKLLEVPMQGISNSSSVYFVSLHSVVKMADVRGLVWGWMNVTDQSAQIVTFTTQADKPCKFPMYRRNFTSIDFQILVPVGCVSSGKEKEKMLRTVYVDEVMDRGPRDRV | MEPLRPPWHLLLLCLCLLSLLPSGFSTEETVSTGPLEKRQLLTRGNIFSQTENLRQGEQDSFPIYVYSLGLGGSPSVQCNVRQGLSWTHEFTVTYTDLVPNGGSCSNFSSMRYHWSRRCSVRVYTLSITGITPSLRYHMKQQGKLLVTLPQQAVQDFPLWLQVNFSDKKLLEVPMQGISNSSSVYFVSLHSVVKMADVRGLVWGWMNVTDQSAQIVTFTTQADKPCKFPMYRRNFTSIDFQILVPVGCVSSGKEKEKMLRTVYYDEVMDRGPRDRV |
Text Diff
--- v3
+++ v4
@@ -1,14 +1,14 @@
-title: TREM2 Ectodomain Variant — Stability Optimized
-quality_score: 0.82
+title: TREM2 Ectodomain Variant — Binding Optimized
+quality_score: 0.88
lifecycle_state: active
-version_tag: stability-opt
-changelog: Introduced 3 stabilizing mutations from Rosetta energy minimization
-metadata.binding_affinity: {'kd_nm': 780, 'target': 'Aβ oligomers'}
-metadata.delta_G_kcal: -2.3
-metadata.design_rationale: Rosetta-guided mutations to improve thermostability without disrupting binding interface
-metadata.method: rosetta_relax
-metadata.mutations: ['T96S', 'K186R', 'A192V']
-metadata.plddt_score: 0.92
-metadata.predicted_stability: 0.91
-metadata.sequence: MEPLRPPWHLLLLCLCLLSLLPSGFSTEETVSTGPLEKRQLLTRGNIFSQTENLRQGEQDSFPIYVYSLGLGGSPSVQCNVRQGLSWTHEFTVTYTDLVPNGGSCSNFSSMRYHRSRRCSVRVYTLSITGITPSLRYHMKQQGKLLVTLPQQAVQDFPLWLQVNFSDKKLLEVPMQGISNSSSVYFVSLHSVVKMADVRGLVWGWMNVTDQSAQIVTFTTQADKPCKFPMYRRNFTSIDFQILVPVGCVSSGKEKEKMLRTVYVDEVMDRGPRDRV
+version_tag: binding-opt
+changelog: Interface mutations to enhance Aβ binding while maintaining stability gains
+metadata.binding_affinity: {'kd_nm': 120, 'target': 'Aβ oligomers'}
+metadata.delta_G_kcal: -1.8
+metadata.design_rationale: Interface residue optimization for enhanced Aβ oligomer recognition. Slight stability trade-off accepted for 6.5x binding improvement.
+metadata.method: rosetta_interface_design
+metadata.mutations: ['T96S', 'K186R', 'A192V', 'H157Y', 'R62W']
+metadata.plddt_score: 0.9
+metadata.predicted_stability: 0.88
+metadata.sequence: MEPLRPPWHLLLLCLCLLSLLPSGFSTEETVSTGPLEKRQLLTRGNIFSQTENLRQGEQDSFPIYVYSLGLGGSPSVQCNVRQGLSWTHEFTVTYTDLVPNGGSCSNFSSMRYHWSRRCSVRVYTLSITGITPSLRYHMKQQGKLLVTLPQQAVQDFPLWLQVNFSDKKLLEVPMQGISNSSSVYFVSLHSVVKMADVRGLVWGWMNVTDQSAQIVTFTTQADKPCKFPMYRRNFTSIDFQILVPVGCVSSGKEKEKMLRTVYYDEVMDRGPRDRV
metadata.uniprot_id: Q9NZC2