Semantic Diff — protein_design-f75a1656-d9fa-4a9f-8aa2-5938e18c516f v5→v4
Summary: score 0.820→0.720 (-0.100); 4 fields changed; 7 metadata keys changed
Changes
| Field | Before | After | Interpretation |
|---|---|---|---|
| title | TREM2 Ectodomain Variant — Stability Optimized | TREM2 Ectodomain Variant — AlphaFold Base | |
| quality_score | 0.82 | 0.72 | |
| version_tag | stability-opt | initial | |
| changelog | Introduced 3 stabilizing mutations from Rosetta energy minimization | None | |
| metadata.binding_affinity | {'kd_nm': 780, 'target': 'Aβ oligomers'} | {'kd_nm': 850, 'target': 'Aβ oligomers'} | |
| metadata.delta_G_kcal | -2.3 | None | |
| metadata.design_rationale | Rosetta-guided mutations to improve thermostability without disrupting binding interface | Wild-type ectodomain structure as baseline for optimization | |
| metadata.method | rosetta_relax | alphafold | |
| metadata.mutations | ['T96S', 'K186R', 'A192V'] | [] | |
| metadata.plddt_score | None | 0.89 | |
| metadata.predicted_stability | 0.91 | 0.72 |
Text Diff
--- v3
+++ v2
@@ -1,13 +1,12 @@
-title: TREM2 Ectodomain Variant — Stability Optimized
-quality_score: 0.82
+title: TREM2 Ectodomain Variant — AlphaFold Base
+quality_score: 0.72
lifecycle_state: active
-version_tag: stability-opt
-changelog: Introduced 3 stabilizing mutations from Rosetta energy minimization
-metadata.binding_affinity: {'kd_nm': 780, 'target': 'Aβ oligomers'}
-metadata.delta_G_kcal: -2.3
-metadata.design_rationale: Rosetta-guided mutations to improve thermostability without disrupting binding interface
-metadata.method: rosetta_relax
-metadata.mutations: ['T96S', 'K186R', 'A192V']
-metadata.predicted_stability: 0.91
+version_tag: initial
+metadata.binding_affinity: {'kd_nm': 850, 'target': 'Aβ oligomers'}
+metadata.design_rationale: Wild-type ectodomain structure as baseline for optimization
+metadata.method: alphafold
+metadata.mutations: []
+metadata.plddt_score: 0.89
+metadata.predicted_stability: 0.72
metadata.sequence: MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG
metadata.uniprot_id: Q9NZC2