Figure 8: 53BP1 forms droplets in vitro in a transcription-dependent manner and 53BP1 LL...

53BP1 forms droplets in vitro in a transcription-dependent manner and 53BP1 LLPS is important for DSB repair in cells. (a) Representative images of droplet formation in U2OS 53BP1-GFP nuclear extract. Both DIC and GFP (FITC) channels are shown. Experiment was repeated twice with similar results. (b) FRAP of internal fraction of a 53BP1-GFP condensate and snapshots of recovery are shown. Experiment was repeated twice with similar results. (c) Representative images of droplet formation in U2OS 53BP1-GFP nuclear extract. H2AX-containing nucleosomes and rNTPs were added to trigger the reaction and immediately treated as indicated. Both DIC and GFP (FITC) channels are shown. N=1. (d) Representative images of droplet formation in U2OS 53BP1-GFP nuclear extract treated as indicated. H2AX-containing nucleosomes and rNTPs were added to trigger the reaction and, after droplets formation, treated as indicated. Both DIC and GFP (FITC) channels are shown. N=1. (e) DNA repair kinetics were monitor

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