Fig. 1: A TREM2 reporter mouse reveals gradual upregulation of TREM2 in microglia. A Sc...

A TREM2 reporter mouse reveals gradual upregulation of TREM2 in microglia. A Schematic of the reporter construct and the proteins expressed in vitro. Created in BioRender. Mühlhofer, M. ( https://BioRender.com/mls61se . B Representative immunofluorescent images of HeLa cells transfected with the reporter construct shown in ( A ). mKate2 and TREM2 stainings are displayed in magenta and green. Scale bars 20 µm and 5 µm. C Immunoblot analysis of time-dependent degradation of TREM2 and mKate2 upon transient overexpression in HeLa cells. Various TREM2 species including the C-terminal fragment (CTF) are indicated. Shown are three technical replicates from a single experiment. Actin was used as a loading control. The asterisk indicates an unspecific signal. Non trans., non-transfected HeLa cells. Uncropped images in Source Data. D Quantification of the immunoblot data shown in ( C ). For TREM2, the immature and mature TREM2 signals were quantified while for mKate2 the two signals below

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