Exploratory experiment designed to discover new patterns targeting PAD2, PAD4 in PAD2/4 deficient mice, human neutrophils. Primary outcome: independence of carbamylation from PAD4, myeloperoxidase, neutrophil elastase, and oxidative pathway
This experiment investigated the enzymatic pathways responsible for histone carbamylation during NETosis using pharmacologic inhibitors and genetically modified mice deficient in peptidylarginine deiminases (PAD2/4). The study tested whether carbamylation was dependent on known NET-associated enzymes including PAD4, myeloperoxidase, neutrophil elastase, or oxidative pathways. Various pharmacological inhibitors were used to block these pathways, and PAD2/4 knockout mice were employed to definitively test the role of these enzymes in the carbamylation process.
pharmacologic inhibitors, genetic knockout mice, carbamylation analysis
determination of enzymatic requirements for histone carbamylation
carbamylation occurs independent of tested enzymatic pathways
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