🧫
Enzymatic Pathway Investigation Using Inhibitors and KO Mice
active
experiment
Created: 2026-04-10T23:14:19
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-d627367a-38cc-4476-9a9a-a59ccf0f0c72
🧫 Experiment Protocol
Exploratoryrheumatoid arthritisPAD2, PAD4PAD2/4 deficient mice, human neutrophilsproposed
This experiment investigated the enzymatic pathways responsible for histone carbamylation during NETosis using pharmacologic inhibitors and genetically modified mice deficient in peptidylarginine deiminases (PAD2/4). The study tested whether carbamylation was dependent on known NET-associated enzymes including PAD4, myeloperoxidase, neutrophil elastase, or oxidative pathways. Various pharmacological inhibitors were used to block these pathways, and PAD2/4 knockout mice were employed to definitively test the role of these enzymes in the carbamylation process.
PRIMARY OUTCOME
independence of carbamylation from PAD4, myeloperoxidase, neutrophil elastase, and oxidative pathways
EXPECTED OUTCOMES
determination of enzymatic requirements for histone carbamylation
SUCCESS CRITERIA
carbamylation occurs independent of tested enzymatic pathways
PROTOCOL
pharmacologic inhibitors, genetic knockout mice, carbamylation analysis
Source: PMID 41930627 ↗
🧫 Experiment Extras
PATHWAY
peptidylarginine deiminase pathway, myeloperoxidase pathway, neutrophil elastase pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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