🧪 Experiments

724 proposed experiments from the Forge — designed to validate, falsify, and explore hypotheses

724
Total Experiments
0.71
Avg Score
0.52
Avg Feasibility
171
Clinical
202
Validation
27
Falsification
All Types animal_model (7)cell_biology (8)clinical (171)computational (12)exploratory (242)falsification (27)gene_expression (6)genetic_association (16)in-vitro (4)in_vitro (2)in-vivo (5)in_vivo (8)neuropathology (14)validation (202)
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clinical 1.00
PANORAMA1: Panobinostat + Bortezomib + Dexamethasone vs Placebo + Bortezomib + Dexamethasone in Relapsed Multiple Myelom
🧬 N/A 🏥 Multiple myeloma
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Randomized, double-blind, placebo-controlled phase 3 trial with 21-day treatment cycles. Panobinostat 20mg orally on day...
Expected: Improved progression-free survival with panobinostat combination based on preclinical synergistic an...
exploratory 0.95
TRIM21-mediated K63-linked ubiquitination of G3BP1
🧬 G3BP1 🏥 N/A 🔗 4 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Materials: Recombinant TRIM21 E3 ligase, purified G3BP1 protein, E1/E2 enzymes (UBE1, UbcH5c), K63-only ubiquitin chains...
Expected: Quantitative predictions: (1) TRIM21 co-IPs with G3BP1 (>5-fold enrichment over IgG control, p<0.01)...
exploratory 0.95
SPP1-mediated microglial synaptic engulfment assay
🧬 SPP1 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Primary Cell Culture and SPP1 Manipulation** — Week 1-2
Expected: 1. Primary: SPP1 knockdown will reduce microglial synaptic engulfment by >40% compared to scrambled ...
validation 0.95
Sevoflurane-induced neurotoxicity (SIN) rat model
🧬 C1qa 🏥 perioperative neurocognitive disorders 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Animal Preparation and Anesthesia Protocol** — Week 1
Expected: 1. Primary: Prolonged sevoflurane will increase MWM escape latency by >50% and reduce probe trial ti...
exploratory 0.95
cGAS/STING pathway activation by TDP-43-released mtDNA
🧬 CGAS 🏥 amyotrophic lateral sclerosis 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: iPSC-derived Motor Neuron Culture and TDP-43 Manipulation** — Week 1-3
Expected: 1. Primary: cGAS/STING inhibition will reduce TDP-43-induced NF-κB activation by >60% (p < 0.01, Coh...
validation 0.95
Bmal1 knockout in mPFC excitatory neurons
🧬 BMAL1,HOMER1A,CAMK2A 🏥 depression 🔗 2 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### PHASE 1: Pre-Surgical Preparation & Baseline Assessment (Weeks 1–2)
Expected: **Conditional Bmal1 knockout efficiency**: Cre-mediated excision will reduce Bmal1 mRNA by **78–85%*...
exploratory 0.95
GWAS meta-analysis of major depressive disorder across three cohorts
🧬 N/A 🏥 major depressive disorder
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Cohort Preparation and Quality Control** — Month 1-2
Expected: 1. Primary: Identify 15-25 genome-wide significant loci (p < 5×10^-8) associated with MDD risk with ...
exploratory 0.95
QTJD effects on macrophage polarization and inflammatory response
🧬 CD86, CXCL10, CD206, ARG1, MRC1 🏥 Mycoplasma pneumoniae pneumonia 🔗 3 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### PHASE 1: Macrophage Isolation and Culture (Day 0-2)
Expected: **CD86 mRNA expression**: QTJD treatment will increase CD86 transcript levels by 2.8 ± 0.4-fold at 5...
exploratory 0.95
Human Reference Interactome (HuRI) mapping
🧬 N/A 🏥 Mendelian diseases
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Human Protein Library Construction and Validation** — Month 1-3
Expected: 1. Primary: Generate >50,000 high-confidence binary protein-protein interactions representing 4-fold...
exploratory 0.95
Double depletion rescue experiment: tau and MAP6 co-depletion
🧬 Mapt, MAP6 🏥 N/A 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Primary Neuronal Culture Preparation and Transfection** — Days 1-7
Expected: 1. Primary: Complete morphological rescue in tau+MAP6 co-depleted neurons, with dendritic complexity...
exploratory 0.95
MSC to chondrocyte mitochondrial transfer quantification
🧬 N/A 🏥 osteoarthritis
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Phase 1: Cell Culture & Preparation (Day 0)
Expected: **Baseline transfer rate at 24h**: 12-18% of chondrocytes will display double-positive mitochondrial...
clinical 0.95
Simvastatin effects on portal hypertension in cirrhotic patients
🧬 N/A 🏥 cirrhotic portal hypertension
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Study Title:** Randomized, Double-Blind, Placebo-Controlled Phase II/III Clinical Trial of Simvastatin for Reduction o...
Expected: **Primary Endpoint:** 22-28% of simvastatin-treated patients will achieve ≥20% HVPG reduction or HVP...
clinical 0.95
Clinical characterization of GABRA1/GABRG2 microdeletion patient
🧬 GABRA1, GABRG2 🏥 epilepsy with optic atrophy 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Phase 1: Initial Clinical Assessment (Day 0)
Expected: **aCGH reveals exact breakpoints:** Microdeletion spans 142-158kb on chromosome 5q34, encompassing G...
clinical 0.95
Phase I trial of NP137 in advanced endometrial cancer
🧬 NTN1 🏥 advanced endometrial carcinoma 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Phase I Trial of NP137 in Advanced Endometrial Cancer
Expected: **DLT Rate:** ≤1/6 patients (16.7%) will experience a dose-limiting toxicity at Dose Level 1 (8 mg/k...
clinical 0.95
FK866 efficacy in IBD patient-derived lamina propria cells
🧬 NAMPT 🏥 Inflammatory bowel disease 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### PHASE 1: Patient Recruitment and Tissue Acquisition (Day -14 to Day 0)
Expected: **FK866 will suppress TNF-α release with IC₅₀ between 1-10 nM.** In IBD patient LPMNCs stimulated wi...
exploratory 0.95
IDH1 and IDH2 mutation sequencing in CNS and non-CNS tumors
🧬 IDH1, IDH2 🏥 gliomas (astrocytomas, oligodendrogliomas, glioblastomas) 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**IDH1 and IDH2 Mutation Sequencing in CNS and Non-CNS Tumors**
Expected: **CNS tumor cohort**: IDH1/IDH2 mutation frequency of 75-85% in astrocytomas (WHO II-IV) and oligode...
validation 0.95
Chi3l1 deletion in APP/PS1 mouse model of Alzheimer's disease
🧬 Chi3l1 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Study Design
Expected: **Amyloid plaque burden (Thioflavin-S)** in APP/PS1;Chi3l1^-/- will be 35–45% lower than APP/PS1;Chi...
clinical 0.95
Wellspent App RCT for Problematic Social Media Use
🧬 N/A 🏥 problematic social media use
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Randomized controlled trial with 1:1 allocation to intervention vs control groups. Intervention group received Wellspent...
Expected: Reduction in problematic social media use, increased self-efficacy, and decreased daily screen time ...
clinical 0.95
ACEs and Motoric Cognitive Risk Syndrome in Chinese Older Adults
🧬 N/A 🏥 motoric cognitive risk syndrome
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Cross-sectional analysis using CHARLS data. Assessment of 12 ACE indicators across categories. MCR diagnosis based on co...
Expected: Higher prevalence of MCR in individuals with more ACEs, with depression and chronic pain serving as ...
clinical 0.95
Preinjury ACE-I/ARB exposure and outcomes in transfused trauma patients
🧬 N/A 🏥 trauma
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Retrospective analysis comparing trauma patients receiving blood transfusions who had preinjury ACE-I/ARB exposure versu...
Expected: Based on previous sepsis research showing improved mortality with ACE-I/ARB use, researchers expecte...
clinical 0.95
Intensive Medical Treatment vs Usual Care in Women with ANOCA/INOCA
🧬 N/A 🏥 angina with no obstructive coronary artery disease (ANOCA/INOCA)
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Randomized controlled trial with participants assigned to intensive medical treatment (high-intensity statin + ACE inhib...
Expected: Reduction in major adverse cardiovascular events with intensive medical treatment compared to usual ...
clinical 0.95
rTMS for preventing chronic postsurgical pain in thoracoscopic surgery
🧬 N/A 🏥 chronic postsurgical pain
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Randomized controlled trial with 286 patients screened, 230 randomized to active or sham rTMS targeting left DLPFC (10 H...
Expected: Reduction in CPSP incidence, improved anxiety and depression scores, decreased inflammatory biomarke...
validation 0.95
Myricanol treatment in HFD-induced hyperlipidemic C57BL/6J mice
🧬 ACSL1, SCD1, PPARγ 🏥 hyperlipidemia
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
8-week HFD induction followed by 25-day treatment with myricanol (100 or 150 mg/kg) or rosiglitazone (0.78 mg/kg). Multi...
Expected: Reduction in serum lipid levels, decreased hepatic lipid accumulation, weight loss, and modulation o...
validation 0.95
PS-NMP shape-dependent colonic barrier damage in mice
🧬 N/A 🏥 intestinal barrier dysfunction
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
120 five-week-old male C57BL/6J mice divided into seven groups and orally exposed to different doses of PS-NMPs (beads a...
Expected: Different shaped PS-NMPs would cause varying degrees of intestinal barrier damage with distinct mole...
validation 0.95
Curcumin-Licorice Combination in D-galactose/Sodium Nitrite AD Model
🧬 N/A 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Mice received D-galactose/sodium nitrite to induce AD pathology, then treated with curcumin (100 mg/kg), G. glabra (100 ...
Expected: Synergistic improvement in cognitive function and reduction in neuroinflammation with combination th...
exploratory 0.95
DPYD polymorphisms association with fluoropyrimidine ADRs
🧬 DPYD 🏥 cancer (patients receiving fluoropyrimidine chemotherapy)
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Real-Time PCR analysis of DNA extracted from 3ml whole blood samples to genotype nine DPYD variants: c.496A>G, c.1236G>A...
Expected: identification of DPYD variants significantly associated with fluoropyrimidine ADRs that could be us...
clinical 0.95
Reproductive fitness analysis of psychiatric disorders in Swedish population
🧬 N/A 🏥 schizophrenia, autism, bipolar disorder, depression, anorexia nervosa, substance abuse
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Population-based analysis using Swedish Multi-Generation Register and Hospital Discharge Register. Measured fecundity of...
Expected: Determine reproductive fitness patterns across psychiatric disorders and evaluate selection pressure...
validation 0.95
Electroacupuncture ST37 treatment for DSS-induced colitis in mice
🧬 CHAT, CHRNA7 🏥 colitis 🔗 3 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Mice received 2.5% DSS solution for 7 days to induce colitis, followed by electroacupuncture treatment at ST37 acupoint....
Expected: EA treatment was expected to and did reduce colitis severity, decrease pro-inflammatory cytokines, a...
clinical 0.95
KEEPS Continuation: Long-term effects of menopausal hormone therapy on AD biomarkers
🧬 APOE 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Participants from the original KEEPS trial were recontacted 10 years after completion. Amyloid beta burden was assessed ...
Expected: The study hypothesized that imaging biomarkers would reveal early detection of evolving brain pathol...
clinical 0.95
Meta-analysis of physical activity interventions on cognitive function in AD
🧬 N/A 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Systematic review and meta-analysis following PRISMA guidelines. Searched PubMed, Web of Science, Embase, Cochrane Libra...
Expected: Physical activity interventions would improve cognitive function as measured by MMSE scores in AD pa...
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