Research Question: The authors identify SPI1 as a potential transcription factor regulating the hub genes but provide no mechanistic details of this regulatory relationship. Given SPI1's role in microglial activation and neuroinflammation, this regulatory circuit may be relevant to cerebrovascular disease and neurodegeneration. Gap type: unexplained_observation Source paper: An integrative analysis of single-cell and bulk transcriptome and bidirectional mendelian randomization analysis identified C1Q as a novel s