Exploratory experiment designed to discover new patterns targeting SARS-CoV-2 spike protein receptor-binding domain in Triple knock-in mice expressing human ACE2, TMPRSS2, and FCGRT. Primary outcome: Loss of protection after RBD antibody depletion
Investigation of the specific contribution of receptor-binding domain (RBD)-targeting antibodies to the protective efficacy of hybrid immunity plasma. The experiment involved depleting RBD-specific antibodies from hybrid immune plasma and testing the remaining plasma for protective capacity against SARS-CoV-2 infection in TKI mice. The depletion of RBD-targeting antibodies completely abrogated the protection provided by hybrid immune plasma, demonstrating that these specific antibodies are crucial for the superior protective efficacy observed with hybrid immunity. This mechanistic study identified RBD-targeting antibodies as the key protective component in hybrid immunity plasma.
Depletion of RBD-targeting antibodies from hybrid immune plasma using specific immunoadsorbent columns, followed by testing of depleted plasma in TKI mouse protection assays
Depletion of RBD antibodies should eliminate the protective capacity of hybrid immunity plasma
Complete loss of protection against viral infection after RBD antibody depletion
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