🧫
TFEB silencing and misfolded protein degradation
active
experiment
Created: 2026-04-06T12:29:04
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2141f24c-6948-4c8b-a156-22051d521fd1
🧫 Experiment Protocol
Exploratorymotoneuron degenerationTFEBNSC34 neuroblastoma x spinal cord cellsproposed
This experiment tested the functional importance of TFEB in trehalose-mediated clearance of misfolded proteins associated with motoneuron diseases. The researchers used siRNA to silence TFEB expression and then measured the ability of trehalose to promote degradation of disease-causing misfolded proteins. This approach directly tested whether TFEB is required for the neuroprotective effects of trehalose in models of motoneuron degeneration.
PRIMARY OUTCOME
misfolded protein clearance
EXPECTED OUTCOMES
TFEB silencing should counteract trehalose's ability to promote misfolded protein degradation
SUCCESS CRITERIA
Significant reduction in trehalose's pro-degradative activity when TFEB is silenced
PROTOCOL
TFEB silencing using siRNA followed by trehalose treatment and measurement of misfolded protein levels using filter retardation assay and immunoblotting
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
protein quality control/autophagy
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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