16S rRNA sequencing of gut microbiome in PH-PS treated mice

This experiment specifically focused on analyzing the gut microbiome changes induced by PH-PS treatment using 16S rRNA gene sequencing. The study examined the intestinal flora composition in 5×FAD mice before and after PH-PS treatment to understand how the polysaccharide modulates gut bacteria populations. The sequencing analysis revealed that PH-PS treatment promoted beneficial probiotic microbiota including Lactobacillus, Muribaculum, Monoglobus, and [Eubacterium]_siraeum_group, while simultaneously suppressing inflammation-related bacteria such as UCG-009 and Blautia. This microbiome remodeling was linked to the restoration of intestinal barrier function and reduction of peripheral inflammation. The experiment provided crucial evidence for the gut-brain axis mechanism underlying PH-PS neuroprotective effects, demonstrating that dietary polysaccharides can influence brain health through modulation of the intestinal microbiome.