🧫
NAD depletion effects on monocyte/macrophage differentiation
active
experiment
Created: 2026-04-10T14:44:04
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-668ff0ed-593d-4597-a9d6-fbd6212e8247
🧫 Experiment Protocol
ExploratoryInflammatory bowel diseaseNAMPTPrimary mouse and human monocytes/macrophagesproposed
In vitro investigation of how NAD depletion affects the differentiation and polarization of primary monocytes and macrophages from both mouse and human sources. The experiment examined the impact of FK866-mediated NAD depletion on macrophage biology, specifically focusing on how reduced NAD availability influences macrophage polarization states. Results showed that NAD depletion skewed macrophage polarization toward an anti-inflammatory phenotype, with reduced expression of pro-inflammatory markers (CD86, CD38, MHC-II, IL-6) and increased expression of anti-inflammatory markers (CD206, Egr2, IL-10). This mechanistic study provided crucial insights into how NAD metabolism directly regulates immune cell function.
PRIMARY OUTCOME
Macrophage polarization markers and cytokine production
EXPECTED OUTCOMES
Altered macrophage polarization with reduced pro-inflammatory and increased anti-inflammatory markers
SUCCESS CRITERIA
Significant changes in polarization markers (CD86, CD38, MHC-II, IL-6 reduction; CD206, Egr2, IL-10 increase)
PROTOCOL
Isolation of primary monocytes/macrophages, FK866 treatment, assessment of differentiation markers and cytokine profiles
LINKED HYPOTHESES
Source: PMID 28877980 ↗
🧫 Experiment Extras
PATHWAY
NAD metabolism, macrophage polarization
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.