🧫
Trehalose-induced lysosomal membrane permeabilization and TFEB activation
active
experiment
Created: 2026-04-06T12:28:58
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-f700c392-bfd0-4fe7-89ac-4e4f696413cb
🧫 Experiment Protocol
Exploratorymotoneuron degenerationTFEBNSC34 neuroblastoma cellsproposed
This experiment investigated the mechanism by which trehalose induces autophagy in neuronal cell models. The study examined trehalose-mediated lysosomal enlargement and membrane permeabilization (LMP), leading to calcium-dependent phosphatase PPP3/calcineurin activation, TFEB dephosphorylation and nuclear translocation. The researchers used multiple cell lines including NSC34 neuroblastoma cells and demonstrated that trehalose upregulates genes for lysosomal components and autophagy-related proteins in a PPP3- and TFEB-dependent manner. The study also tested trehalase-resistant analogs like melibiose and lactulose to confirm the mechanism is independent of trehalose metabolism.
PRIMARY OUTCOME
TFEB nuclear translocation and autophagy gene upregulation
EXPECTED OUTCOMES
Trehalose treatment would induce lysosomal membrane permeabilization, activate calcineurin, promote TFEB nuclear translocation, and upregulate autophagy genes
SUCCESS CRITERIA
Significant increase in TFEB nuclear localization, upregulation of autophagy-related genes, and enhanced clearance of misfolded proteins
PROTOCOL
Cell culture treatment with trehalose and analogs, immunofluorescence microscopy, western blotting, RT-qPCR, lysosomal membrane permeabilization assays, calcium imaging, siRNA knockdown studies
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
autophagy-lysosomal pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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