experiment 1,004 words KG: ent-gene-95250f02 2026-03-25
kind:experimentsection:experimentsstate:publishedparkinsoncgas-stinginterferon
Contents

cGAS-STING Pathway Validation Study in Parkinson's Disease

🔬 Protein Info
Gene Symbolent-gene-95250f02
ModeliPSC-derived microglia + dopaminergic neurons from PD patients (LRRK2 G2019S, idiopathic, GBA carriers) and healthy controls
Condition GroupsControl, PD neurons only, PD neurons + cGAS activators (dsDNA transfection), PD neurons + cGAS-STING inhibitors (G150, H151)
Readouts- Behavioral: cylinder test, stepping test, gait analysis, rotarod - Biochemical: striatal dopamine, TH+ neuron counts in SNc - Molecular: cGAS-STING pathway activation (cGAMP, p-STING) - Type I IFN markers: IFN-β, ISG56 - Inflammation: Iba1+ microglial density, cytokine levels - α-synuclein pathology: pSer129 burden
ModelsMPTP-induced PD model, α-synuclein pre-formed fibril (PFF) model, PINK1 knockout (mtDNA release model)
Treatment GroupsVehicle, cGAS inhibitor (G150, 30mg/kg i.p. daily), STING inhibitor (H151, 10mg/kg i.p. daily), Positive control (L-DOPA)
Duration52 weeks
Cohort400 PD patients (various stages), 150 controls
SamplesCSF, plasma, peripheral blood mononuclear cells (PBMCs)
BiomarkerscGAMP (CSF and plasma), STING, p-STING, IFN-β, ISG56, CXCL10 in CSF; genetic variants in cGAS/STING genes
CorrelationUPDRS motor score, MoCA, DAT-SPECT imaging, disease progression rate
DesignRandomized, double-blind, placebo-controlled
Primary EndpointBiomarker levels vs. clinical measures
SecondaryBiomarker changes over 24-month follow-up
PopulationEarly-stage PD (Hoehn & Yahr 1-2) with elevated cGAMP or IFN markers
InterventionSTING inhibitor (to be determined based on BBB penetration) or placebo
KG Connections2 knowledge graph edges
DatabasesGeneCardsUniProtNCBI GeneHPASTRING

Knowledge Graph

Related Hypotheses (8)

Bacterial Enzyme-Mediated Dopamine Precursor Synthesis
Score: 0.36
Senescent Cell Mitochondrial DNA Release
Score: 0.54
cGAS-STING Senescence Circuit Disruption
Score: 0.49
Circadian-Synchronized LRP1 Pathway Activation
Score: 0.53
Astrocytic Lipoxin A4 Pathway Restoration via ALOX15 Gene Th
Score: 0.45

Related Analyses (23)

What determines the specificity of TDP-43-induced mitochondr
neurodegeneration · failed
How does chronic cGAS/STING activation downstream of TDP-43
neuroinflammation · failed
What upstream mechanisms cause TDP-43 to trigger mPTP openin
neurodegeneration · failed
Astrocyte reactivity subtypes in neurodegeneration
neurodegeneration · archived
Blood-brain barrier transport mechanisms for antibody therap
neurodegeneration · archived

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Knowledge Graph (2 edges)

ent-gene-95250f02 data_in benchmark_ot_ad_answer_key:CGAS
benchmark_ot_ad_answer_key:CGAS data_in ent-gene-95250f02