TUG C-terminal product nuclear localization and PPARγ binding

Exploratory Score: 0.850 Price: $0.50 Metabolic regulation Cell culture and mouse tissue Status: proposed

What This Experiment Tests

Exploratory experiment designed to discover new patterns targeting Tug (Aspscr1) in Cell culture and mouse tissue. Primary outcome: Nuclear localization and protein-protein interactions

Description

Investigation of the mechanism by which the TUG C-terminal cleavage product enters the nucleus and interacts with PPARγ and PGC-1α. This study demonstrated that after insulin-stimulated cleavage and GLUT4 release, the TUG C-terminal fragment translocates to the nucleus where it binds to transcriptional regulators to control gene expression programs involved in lipid oxidation and thermogenesis.

TARGET GENE

Tug (Aspscr1)

MODEL SYSTEM

Cell culture and mouse tissue

ESTIMATED COST

TIMELINE

0 months

PATHWAY

PPARγ/PGC-1α transcriptional regulation

SOURCE

extracted_from_pmid_33686286

PRIMARY OUTCOME

Nuclear localization and protein-protein interactions

Scoring Dimensions

📖 Wiki Pages

PGC-1α (PPARGC1A) Targeted Therapies in Neurodegentherapeutic PGC-1α Activator Therapy for Neurodegenerationtreatment PGC-1β Proteinprotein PGC-1 Alphaprotein PGC-1α (PPARGC1A)protein PGC-1α Proteinredirect PGC-1α Proteinprotein PGC-1α and Mitochondrial Biogenesis Therapies for therapeutic PGC-1α Mitochondrial Biogenesis Comparison — AD/PDmechanism

Protocol

Subcellular fractionation, immunofluorescence microscopy, co-immunoprecipitation, chromatin immunoprecipitation

Expected Outcomes

TUG C-terminal product localizes to nucleus and binds PPARγ/PGC-1α

Success Criteria

Demonstration of nuclear localization and specific protein interactions

Related Hypotheses (0)

No related hypotheses

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