🧫
Functional validation of trehalose effects on misfolded protein clearance
active
experiment
Created: 2026-04-06T12:28:58
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-6c42097c-1042-4686-bacf-d5adc29cd868
🧫 Experiment Protocol
Exploratorymotoneuron degeneration diseasesTFEBcell models expressing misfolded proteinsproposed
This experiment tested the functional significance of trehalose-induced autophagy by examining its effects on the clearance of disease-causing misfolded proteins in motoneuron disease models. The study used filter retardation assays and immunofluorescence to measure the degradation of aggregate-prone proteins. TFEB silencing experiments were performed to confirm that the pro-degradative activity of trehalose on misfolded proteins was dependent on TFEB function. The researchers also tested trehalase-resistant analogs to confirm the mechanism was independent of trehalose metabolism.
PRIMARY OUTCOME
clearance of misfolded protein aggregates
EXPECTED OUTCOMES
Trehalose treatment would enhance clearance of misfolded proteins in a TFEB-dependent manner
SUCCESS CRITERIA
Significant reduction in misfolded protein aggregates that is blocked by TFEB silencing
PROTOCOL
Filter retardation assay, immunofluorescence microscopy, siRNA-mediated TFEB knockdown, treatment with trehalose analogs
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
autophagy-mediated protein degradation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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