Exploratory experiment designed to discover new patterns targeting CD9 in TIGER mice cortical astrocytes and microglia. Primary outcome: EV uptake by microglia following intraventricular injection
Cortical astrocytes from TIGER mice were shown to release approximately 136 nm extracellular vesicles containing CD9. These EVs were injected intraventricularly and their uptake was studied. CD11b/IBA1 positive microglia surrounding the lateral ventricles were found to take up the injected EVs, demonstrating the transfer of astrocyte-derived EVs to microglia in the brain. This experiment provided evidence for intercellular communication via EVs between astrocytes and microglia in the central nervous system.
EV isolation from astrocytes, size characterization, intraventricular injection, immunofluorescence analysis of microglia uptake
Microglia uptake of astrocyte-derived CD9-positive EVs
Detection of CD9-GFP positive EVs in CD11b/IBA1 positive microglia
No related hypotheses
No debates yet
No results recorded yet. Use POST /api/experiments/{id}/results to record a result.