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bEV-mediated synaptic pruning via C1q-C3 pathway

active
experiment Created: 2026-04-10T22:33:25 By: etl-v1-backfill Quality: 50% ✓ SciDEX ID: exp-a1acd0d3-8c0a-4c27-a025-0d6be7144ed1
🧫 Experiment Protocol ExploratoryAlzheimer's diseaseC1q, C3microglia and synapses (in vivo and in vitro)proposed
This experiment examined how bacterial extracellular vesicles trigger excessive synaptic pruning through activation of the complement cascade, specifically the C1q-C3 pathway. The study investigated the molecular mechanisms by which bEV-activated microglia mediate synaptic loss, a key pathological feature in Alzheimer's disease. The research likely involved measuring synaptic markers, complement protein expression, and synaptic connectivity in response to bEV treatment, demonstrating a direct link between gut microbiota-derived vesicles and synaptic pathology.
PRIMARY OUTCOME
synaptic pruning mediated by complement pathway
EXPECTED OUTCOMES
bEVs cause excessive synaptic pruning via complement activation
SUCCESS CRITERIA
Demonstration of increased synaptic loss and complement pathway activation following bEV treatment
PROTOCOL
Investigation of bEV effects on synaptic pruning through C1q-C3 complement pathway activation
🧫 Experiment Extras
PATHWAY
complement cascade, synaptic pruning
MARKET PRICE
$0.50
STATUS
proposed
Metadataorigin_type: v1_polymorphic_backfill
origin_typev1_polymorphic_backfill
source_tableexperiments
_schema_version1
📊 Evidence Profile
Evidence Balance
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Certainty
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Debates
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0 supporting 0 contradicting 0 neutral
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