🧪 Experiments

8 proposed experiments from the Forge — designed to validate, falsify, and explore hypotheses

8
Total Experiments
0.71
Avg Score
0.52
Avg Feasibility
171
Clinical
202
Validation
27
Falsification
All Types animal_model (7)cell_biology (8)clinical (171)computational (12)exploratory (242)falsification (27)gene_expression (6)genetic_association (16)in-vitro (4)in_vitro (2)in-vivo (5)in_vivo (8)neuropathology (14)validation (202)
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in_vivo 0.80
nSMase2 Inhibitor DPTIP Rescues Synaptic Density in APPswe/PS1deltaE9 Mice
🧬 SMPD3 🏥 Alzheimer's disease 🔗 1 hyp
💰 $62,000 ⏱ 14mo ✅ 0.74 ✨ 0.80
Treat APP/PS1 mice with DPTIP (10 mg/kg i.p., daily) or vehicle for 12 weeks (6-9 months). 2. Weekly body weight, open f...
Expected: DPTIP will reduce ceramide Cer16:0 by >=60%, cut exosome secretion by >=40%, restore PSD-95 to withi...
in_vivo 0.79
TREM2 Agonist Antibody Rescue of Astrocyte-Microglia Signalling in 5xFAD Mice
🧬 TREM2 🏥 Alzheimer's disease 🔗 1 hyp
💰 $85,000 ⏱ 18mo ✅ 0.72 ✨ 0.78
Breed 5xFAD hemizygous x C57BL/6J; genotype at P21. 2. Randomise to 4 groups at 3 months (n=15/group, sex-balanced). 3. ...
Expected: AL002c-analogue will suppress astrocyte A1 conversion by >=40% (C3, Lcn2), shift microglia toward DA...
in_vivo 0.77
Anti-miR-155 LNA Oligonucleotide Shifts Microglia to Homeostatic State in LPS-Challenged and 3xTg-AD Mice
🧬 MIR155 🏥 neurodegeneration 🔗 1 hyp
💰 $58,000 ⏱ 14mo ✅ 0.65 ✨ 0.82
Synthesise LNA-155 (5-T+G+T+A+G+C+A+T+T+A+A-3) or scramble-LNA. 2. Intrathecal implant osmotic pump (2 ug/day, 28-day re...
Expected: LNA-155 will reduce microglial miR-155 >=60%, suppress IFN-gamma/IL-6 in CSF >=40%, increase SHIP1 p...
in_vivo 0.77
40 Hz Auditory + Visual Gamma Stimulation Reduces Amyloid and Entrains LFP in 5xFAD Mice
🧬 N/A 🏥 Alzheimer's disease 🔗 1 hyp
💰 $72,000 ⏱ 14mo ✅ 0.68 ✨ 0.79
Implant tetrode LFP arrays in CA1 of 5xFAD and WT mice at 4 months. 2. Recovery 1 week; then 4 treatment groups: WT-sham...
Expected: Combined stimulation will entrain CA1 gamma >=2x baseline, reduce soluble Abeta42 >=40%, activate mi...
in_vivo 0.77
Sequential DFO + Sulforaphane Prevents Iron-Ferroptosis Cascade in Rat Cardiac-Arrest Model
🧬 GPX4 🏥 post-cardiac-arrest neurodegeneration 🔗 1 hyp
💰 $48,000 ⏱ 12mo ✅ 0.72 ✨ 0.74
Asphyxial CA: 6-min KCl arrest + CPR in adult SD rats. 2. Groups: sham, CA-vehicle, CA-DFO (100 mg/kg IV at ROSC), CA...
Expected: DFO+SFN will blunt the iron surge (CSF ferritin <=50% of vehicle), maintain GPX4 activity, reduce MR...
in_vivo 0.77
Closed-loop tFUS Enhances Glymphatic Clearance and Restores CA1 PV Interneuron Gamma in APP/PS1 Mice
🧬 PVALB 🏥 Alzheimer's disease 🔗 1 hyp
💰 $125,000 ⏱ 20mo ✅ 0.55 ✨ 0.87
APP/PS1 mice, 8 months, MRI-guided tFUS targeting dorsal CA1. 2. Closed-loop: slow-oscillation (0.5-1 Hz) detection -> t...
Expected: tFUS will increase glymphatic influx 2x (gadolinium signal), reduce PV-soma-adjacent Abeta >=35%, re...
in_vivo 0.75
Conditional TYROBP Knockdown in Early-Stage 5xFAD Mice Using CRISPRi-AAV9
🧬 TYROBP 🏥 Alzheimer's disease 🔗 1 hyp
💰 $95,000 ⏱ 20mo ✅ 0.55 ✨ 0.84
Construct AAV9-flex-dCas9-KRAB with 3 sgRNAs targeting TYROBP exon 2. 2. ICV injection at P60 (2 uL, 5e12 vg/mL) in 5xFA...
Expected: CRISPRi will reduce TYROBP 50-70%, shift microglia away from DAM-high inflammatory states, preserve ...
in_vivo 0.74
tFUS Targeting EC-II SST Interneurons Restores Hippocampal-Prefrontal Gamma Sync in hAPP-J20 Mice
🧬 SST 🏥 Alzheimer's disease 🔗 1 hyp
💰 $110,000 ⏱ 22mo ✅ 0.48 ✨ 0.88
Implant LFP/unit recording arrays in EC-II, CA1, mPFC at 3 months hAPP-J20. 2. Closed-loop tFUS system: real-time theta ...
Expected: Closed-loop tFUS will increase gamma coherence >=2x, drive SST interneuron firing phase-locked to th...
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