An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues.
Corces M Ryan; Trevino Alexandro E; Hamilton Emily G; Greenside Peyton G; Sinnott-Armstrong Nicholas A; Vesuna Sam; Satpathy Ansuman T; Rubin Adam J; Montine Kathleen S; Wu Beijing; Kathiria Arwa; Cho Seung Woo; Mumbach Maxwell R; Carter Ava C; Kasowski Maya; Orloff Lisa A; Risca Viviana I; Kundaje Anshul; Khavari Paul A; Montine Thomas J; Greenleaf William J; Chang Howard Y
Nature methods 2017
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.