🧫
LPS-induced mouse model of depression with synaptic loss analysis
active
experiment
Created: 2026-04-10T14:35:54
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-126e411e-2d1d-47f8-b220-3b2edcd75f48
🧫 Experiment Protocol
ValidationdepressionC1qLPS-treated miceproposed
This experiment used lipopolysaccharide (LPS) administration to induce a mouse model of depression. Researchers examined the effects of LPS on multiple parameters including depressive and anxiety-like behaviors, synaptic integrity, microglial activation, and microglial phagocytosis of synapses in the hippocampal dentate gyrus. The study focused on understanding how activated microglia dysfunctionally engulf neuronal synapses under depressive conditions, leading to synaptic loss and behavioral impairments. The complement C1q/C3-CR3 signaling pathway was investigated as a potential mediator of this abnormal microglial-synaptic interaction. Behavioral assessments were conducted to evaluate depression and anxiety-like phenotypes, while histological and molecular analyses were performed to assess synaptic density, microglial activation status, and phagocytic activity.
PRIMARY OUTCOME
depressive and anxiety-like behaviors, synaptic loss, abnormal microglial phagocytosis
EXPECTED OUTCOMES
LPS treatment would induce depressive behaviors, activate microglia, increase synaptic phagocytosis, and activate complement signaling
SUCCESS CRITERIA
demonstration of behavioral deficits, synaptic loss, and increased microglial phagocytosis in LPS-treated mice
PROTOCOL
LPS administration to mice followed by behavioral testing, histological analysis of hippocampal dentate gyrus, assessment of microglial phagocytosis of synapses, and evaluation of C1q/C3-CR3 signaling pathway components
LINKED HYPOTHESES
Source: PMID 38642614 ↗
🧫 Experiment Extras
PATHWAY
C1q/C3-CR3 complement signaling pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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