🧫
Cell type-specific P2rx7 deletion in microglia vs astrocytes
active
experiment
Created: 2026-04-12T18:03:52
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2aa445e2-aed9-4ecd-ad42-d526ab36cb8d
🧫 Experiment Protocol
ValidationAlzheimer's disease/tauopathyP2rx7PS19 mice with cell type-specific P2rx7 deletionproposed
This experiment used cell type-specific deletion approaches to determine which cell types mediate P2rx7's effects on tau propagation. Researchers created mice with P2rx7 specifically deleted in either microglia or astrocytes and examined tau propagation from the entorhinal cortex to CA1 region of the hippocampus in PS19 tauopathy mice. This experiment was designed to identify the cellular mechanisms by which P2rx7 contributes to early AD pathology, specifically the spread of tau pathology between brain regions. The study likely used Cre-lox technology to achieve cell type-specific gene deletion.
PRIMARY OUTCOME
Tau propagation from entorhinal cortex to CA1 hippocampus
EXPECTED OUTCOMES
Microglial P2rx7 deletion would suppress tau propagation more than astrocytic deletion
SUCCESS CRITERIA
Significant reduction in tau propagation with microglial but not astrocytic P2rx7 deletion
PROTOCOL
Cell type-specific P2rx7 deletion using Cre-lox system, followed by tau pathology assessment in specific brain regions
LINKED HYPOTHESES
Source: PMID 40678243 ↗
🧫 Experiment Extras
PATHWAY
Cell type-specific P2RX7 signaling in microglia and astrocytes
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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