🧫
TUG C-terminal fragment nuclear interactions
active
experiment
Created: 2026-04-06T12:32:55
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-4aac1248-f5df-4020-afd7-010395f5c91b
🧫 Experiment Protocol
Exploratorymetabolic regulationAspscr1/TUGcell culture systems and mouse tissuesproposed
Investigation of how the TUG C-terminal cleavage product enters the nucleus and interacts with transcriptional regulators. The study examined binding interactions between the TUG cleavage product and peroxisome proliferator-activated receptor (PPAR)γ and its coactivator PGC-1α. Researchers used biochemical approaches to demonstrate direct protein-protein interactions and functional consequences for gene transcription, particularly genes involved in lipid oxidation and thermogenesis.
PRIMARY OUTCOME
protein-protein interactions and transcriptional activity
EXPECTED OUTCOMES
Direct binding between TUG fragments and PPARγ/PGC-1α, enhanced transcriptional activity
SUCCESS CRITERIA
Demonstrated protein interactions and increased transcription of target genes
PROTOCOL
Nuclear fractionation, co-immunoprecipitation, transcriptional reporter assays, chromatin immunoprecipitation
Source: PMID 33686286 ↗
🧫 Experiment Extras
PATHWAY
PPARγ/PGC-1α transcriptional regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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