🧫
In vitro DDR reconstitution on nucleosomes
active
experiment
Created: 2026-04-06T12:31:13
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-532caefb-6973-4955-abb8-1733672f28dc
🧫 Experiment Protocol
Exploratoryin vitro nucleosome systemproposed
This experiment utilized an in vitro system to reconstitute DNA damage response events on nucleosomal substrates. The researchers developed a cell-free system that could recapitulate DDR focus formation and tested the requirement for transcriptional factors in this simplified system. This approach allowed for more controlled analysis of the molecular mechanisms underlying DDR focus formation without the complexity of intact cellular systems.
PRIMARY OUTCOME
DDR event reconstitution in vitro
EXPECTED OUTCOMES
Successful reconstitution of DDR events dependent on transcriptional machinery
SUCCESS CRITERIA
Formation of DDR-like structures in vitro that require transcriptional factors
PROTOCOL
Cell-free reconstitution of DDR events on nucleosomes with controlled addition/removal of transcriptional factors
Source: PMID 31570834 ↗
🧫 Experiment Extras
PATHWAY
DNA damage response pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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