🧫
Silibinin-induced G2/M cell cycle arrest in breast cancer cells
active
experiment
Created: 2026-04-11T00:47:39
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-61d9f784-c4cc-4c26-b175-2968df276bff
🧫 Experiment Protocol
Exploratorybreast cancerYAP1, CAPZA1MCF-7 and MDA-MB-231 human breast cancer cell linesproposed
This experiment investigated the mechanism by which silibinin induces cell cycle arrest in breast cancer cells. The study examined both hormone-sensitive MCF-7 and triple-negative MDA-MB-231 breast cancer cells to determine how silibinin treatment affects cell cycle progression. The researchers found that silibinin induces G2/M phase arrest in both cell lines through inhibition of the YAP pathway. Additionally, they discovered that silibinin significantly reduces F-actin assembly, which is positively regulated by YAP through transcriptional control of polymerization factors. The study demonstrated that F-actin disassembly is not merely a consequence of YAP inhibition but actively contributes to cell cycle arrest. Using molecular techniques including siRNA transfection and cytoskeletal disruption with Cytochalasin D, they showed that F-actin assembly disturbance directly contributes to cell cycle arrest independent of YAP activity.
PRIMARY OUTCOME
G2/M cell cycle arrest and F-actin disassembly
EXPECTED OUTCOMES
Silibinin treatment expected to induce cell cycle arrest through YAP inhibition; discovered that F-actin disassembly is a critical mediator of this effect
SUCCESS CRITERIA
Demonstration of G2/M arrest, reduced F-actin assembly, and restoration of cell cycle progression upon F-actin stabilization
PROTOCOL
Cell culture, silibinin treatment, cell cycle analysis, F-actin staining and microscopy, siRNA transfection (si-YAP/TAZ, si-Capza1), Cytochalasin D treatment, qRT-PCR, protein analysis
Source: PMID 41349910 ↗
🧫 Experiment Extras
PATHWAY
YAP signaling, actin cytoskeleton organization, cell cycle regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.