🧫
iPSC-NPC transplantation in ICH animal model
active
experiment
Created: 2026-04-10T23:10:15
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-778c1bc2-7c6e-4cdd-81af-56c535ead1e3
🧫 Experiment Protocol
Validationintracerebral hemorrhageAMPK, mTORanimal ICH modelproposed
This experiment evaluated the therapeutic effects of induced pluripotent stem cell-derived neural progenitor cell (iPSC-NPC) transplantation in an in vivo intracerebral hemorrhage (ICH) model. The study investigated how iPSC-NPCs influence the AMPK/mTOR signaling pathway and autophagy regulation in the context of stroke recovery. Researchers analyzed protein and mRNA expression changes of key markers including GFAP (glial fibrillary acidic protein), AMPK, mTOR, SQSTM1/P62, and LC3 (microtubule-associated protein 1 light chain 3) using immunofluorescence techniques. The experiment demonstrated that iPSC-NPCs secrete cytokines that mitigate brain injury and protect astrocytes from autophagy by promoting astrocyte activation through inhibiting AMPK phosphorylation and promoting mTOR activation.
PRIMARY OUTCOME
therapeutic effects of iPSC-NPC transplantation
EXPECTED OUTCOMES
iPSC-NPCs would promote behavioral recovery and neural connectivity
SUCCESS CRITERIA
reduced brain injury, astrocyte protection from autophagy, modulation of AMPK/mTOR pathway
PROTOCOL
iPSC-NPC transplantation followed by analysis of protein and mRNA expression, immunofluorescence of GFAP, AMPK, mTOR, SQSTM1/P62, and LC3
LINKED HYPOTHESES
Source: PMID 41921697 ↗
🧫 Experiment Extras
PATHWAY
AMPK/mTOR signaling pathway, autophagy regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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