🧫
Pharmacokinetic evaluation of humanized mAbs in IgG1-Fc humanized mice
active
experiment
Created: 2026-04-10T14:43:28
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-8790de69-970d-4cd8-ae9c-9396a401c029
🧫 Experiment Protocol
ValidationFCGRTIgG1-Fc humanized hFCGRT transgenic miceproposed
This experiment evaluated the pharmacokinetic behavior of administered humanized monoclonal antibodies in the newly created IgG1-Fc humanized mice compared to control conditions. The study demonstrated that endogenous chimeric IgG1 produced by the engineered mice significantly dampened the serum half-life of administered humanized mAbs in an hFCGRT-dependent manner. This competitive effect was designed to more accurately model the human physiological condition where endogenous human IgG competes with therapeutic antibodies for FcRn binding, providing a more translationally relevant preclinical model for human IgG-based biologics development.
PRIMARY OUTCOME
Serum half-life of administered humanized monoclonal antibodies
EXPECTED OUTCOMES
Reduced serum half-life of administered humanized mAbs due to competition with endogenous chimeric IgG1
SUCCESS CRITERIA
Significant dampening of humanized mAb serum half-life in an hFCGRT-dependent manner
PROTOCOL
Administration of humanized mAbs to IgG1-Fc humanized mice followed by pharmacokinetic analysis and comparison to control conditions
Source: PMID 33025844 ↗
🧫 Experiment Extras
PATHWAY
FcRn-mediated IgG recycling and pharmacokinetics
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.