🧫
Ketogenic diet fat threshold analysis in wild-type mice
active
experiment
Created: 2026-04-12T17:54:06
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-8d2aa84d-041e-4491-ac08-185930eb64be
🧫 Experiment Protocol
Validationmetabolic diseaseHmgcs2, Cd36, Cpt1a, Acat1C57BL/6J wild-type miceproposed
This experiment evaluated multiple ketogenic diet formulations with varying fat content (80-95%) in C57BL/6J wild-type mice to determine the fat threshold required for sustained ketosis. Researchers measured plasma, hepatic, and intestinal β-hydroxybutyrate (BHB) levels and assessed expression of ketogenesis and fatty acid oxidation genes including Cd36, Cpt1a, Acat1, and Hmgcs2. Body weight, adipose distribution, and liver morphology were monitored. The study found that only diets exceeding 85% fat induced robust ketogenesis with elevated BHB and hepatic upregulation of key metabolic genes, while moderate KDs (80-85%) failed to trigger ketosis and resembled standard high-fat feeding.
PRIMARY OUTCOME
β-hydroxybutyrate levels and ketogenesis gene expression
EXPECTED OUTCOMES
Identification of fat threshold for sustained ketosis and associated metabolic changes
SUCCESS CRITERIA
Elevated BHB levels and upregulation of ketogenesis genes
PROTOCOL
Multiple KD formulations (80-95% fat) feeding with measurement of plasma, hepatic, and intestinal BHB levels and gene expression analysis
LINKED HYPOTHESES
Source: PMID 41156456 ↗
🧫 Experiment Extras
PATHWAY
ketogenesis, fatty acid oxidation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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