🧫
E2F regulation of endocycles in hepatocytes
active
experiment
Created: 2026-04-06T12:29:34
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-903cf1dd-d4e8-488e-ba1f-54b04fd49832
🧫 Experiment Protocol
ValidationE2F1, E2F2, E2F3, E2F7, E2F8lineage-specific cre mice, liver hepatocytesproposed
This experiment investigated the role of E2F transcription factors in regulating endocycles in liver hepatocytes, the second major endocycling tissue in mammals. Using the same lineage-specific cre mouse system, researchers examined how genetic ablation of canonical E2F activators (E2F1, E2F2, E2F3) versus atypical E2F repressors (E2F7, E2F8) affects hepatocyte ploidy. Hepatocytes naturally undergo endoreduplication during liver development and regeneration, making them an important model for studying endocycle regulation. The study measured genome ploidy changes following selective knockout of different E2F family members to understand their opposing roles in endocycle control.
PRIMARY OUTCOME
genome ploidy levels
EXPECTED OUTCOMES
canonical E2F activator ablation increases ploidy, atypical E2F repressor ablation decreases ploidy
SUCCESS CRITERIA
measurable changes in genome ploidy following E2F gene ablation
PROTOCOL
lineage-specific cre-mediated gene ablation in hepatocytes, ploidy measurement
Source: PMID 23064266 ↗
🧫 Experiment Extras
PATHWAY
E2F transcriptional program, cell cycle regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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