🧫
PPP3/calcineurin-dependent TFEB dephosphorylation mechanism
active
experiment
Created: 2026-04-06T12:28:58
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-ad6850c9-f4ec-48ac-9df6-a7f9a1c8739b
🧫 Experiment Protocol
Exploratorymotoneuron degenerationPPP3CBneuronal cell linesproposed
This experiment investigated the molecular mechanism linking lysosomal membrane permeabilization to TFEB activation. The study examined calcium-dependent phosphatase PPP3/calcineurin activation following trehalose-induced lysosomal damage, leading to TFEB dephosphorylation and subsequent nuclear translocation. The researchers used calcium imaging, phosphatase activity assays, and immunofluorescence to demonstrate that lysosomal calcium release activates calcineurin, which then dephosphorylates TFEB, allowing its nuclear translocation and transcriptional activity.
PRIMARY OUTCOME
TFEB phosphorylation status and nuclear localization
EXPECTED OUTCOMES
Lysosomal calcium release would activate calcineurin, leading to TFEB dephosphorylation and nuclear translocation
SUCCESS CRITERIA
Demonstration of calcium-dependent TFEB dephosphorylation and nuclear translocation that can be blocked by calcineurin inhibitors
PROTOCOL
Calcium imaging, immunofluorescence microscopy, western blotting for phospho-TFEB, subcellular fractionation, phosphatase activity assays
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
calcium-calcineurin-TFEB signaling pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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