🧫
Abeta-induced cell death and aggregation in GD3S-deficient neurons
active
experiment
Created: 2026-04-10T22:32:02
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-afe1d470-63a3-44e9-b882-44f89779a907
🧫 Experiment Protocol
ExploratoryAlzheimer's diseaseSt8sia1primary neurons and astrocytes from GD3S-/- miceproposed
Primary neurons and astrocytes lacking GD3 synthase (GD3S) were cultured and exposed to amyloid-beta peptides to examine cell death and aggregation patterns. The experiment investigated whether the absence of GD3S, which is responsible for biosynthesis of b- and c-series gangliosides, would affect Abeta-induced cellular toxicity and peptide aggregation. Cells were assessed for viability and Abeta aggregation formation compared to wild-type controls. This in vitro study provided mechanistic insights into how ganglioside deficiency might protect against Abeta-mediated neurotoxicity.
PRIMARY OUTCOME
Abeta-induced cell death and aggregation inhibition
EXPECTED OUTCOMES
Reduced Abeta-induced cell death and aggregation in GD3S-deficient cells
SUCCESS CRITERIA
Significant reduction in cell death and Abeta aggregation compared to wild-type controls
PROTOCOL
Primary cell culture from GD3S knockout mice, Abeta exposure, cell viability and aggregation assays
LINKED HYPOTHESES
Source: PMID 18258340 ↗
🧫 Experiment Extras
PATHWAY
ganglioside biosynthesis pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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