🧫
Irisin effects on osteogenic differentiation in mesenchymal stem cells
active
experiment
Created: 2026-04-10T14:42:16
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-db3f412b-7d9b-493e-a89c-ca2db30ee400
🧫 Experiment Protocol
Exploratorybone fractureOSX, Runx2, OPG, ALP, OCN, BMP2mouse bone mesenchymal stem cellsproposed
In vitro study investigating the effects of irisin on osteogenic differentiation using mouse bone mesenchymal stem cells. The experiment evaluated osteogenic differentiation through multiple assays including quantitative real-time polymerase chain reaction (qRT-PCR), alkaline phosphatase (ALP) staining, and alizarin red staining for mineralization. Gene expression analysis focused on key osteogenic markers including OSX, Runx2, OPG, ALP, OCN, and BMP2. Results demonstrated that irisin treatment promoted ALP expression and mineralization, and significantly increased expression of multiple osteogenic genes, indicating enhanced osteogenic differentiation capacity of mesenchymal stem cells when exposed to irisin.
PRIMARY OUTCOME
osteogenic differentiation markers and gene expression
EXPECTED OUTCOMES
enhanced osteogenic differentiation with irisin treatment
SUCCESS CRITERIA
increased ALP expression, mineralization, and osteogenic gene expression
PROTOCOL
qRT-PCR, alkaline phosphatase staining, alizarin red staining for mineralization
LINKED HYPOTHESES
Source: PMID 36196152 ↗
🧫 Experiment Extras
PATHWAY
osteogenic differentiation pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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