🧫
40 Hz Auditory + Visual Gamma Stimulation Reduces Amyloid and Entrains LFP in 5xFAD Mice
active
experiment
Created: 2026-04-26T13:48:17
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-proposal-a50e9ae2d502
🧫 Experiment Protocol
In VivoAlzheimer's disease5xFAD mice 4-6 months, LFP implant (hippocampus CA1), n=10/group x 4 groupsproposed
Determine whether combined 40 Hz auditory (click train) + visual (flickering LEDs) gamma entrainment, 1 h/day x 4 weeks, reduces soluble Abeta and tau in hippocampus and entrains LFP gamma power >=2-fold above baseline in 5xFAD mice.
PRIMARY OUTCOME
Hippocampal soluble Abeta42 reduced >=40% vs sham; CA1 LFP 40 Hz power >=2x baseline
EXPECTED OUTCOMES
Combined stimulation will entrain CA1 gamma >=2x baseline, reduce soluble Abeta42 >=40%, activate microglia phagocytosis, and improve NOR discrimination index.
SUCCESS CRITERIA
Primary: Abeta42 ELISA >=40% reduction vs 5xFAD-sham (Student t, p<0.05). Secondary: LFP 40 Hz power >=2x baseline during stimulation; NOR DI >=0.65.
PROTOCOL
1. Implant tetrode LFP arrays in CA1 of 5xFAD and WT mice at 4 months. 2. Recovery 1 week; then 4 treatment groups: WT-sham, 5xFAD-sham, 5xFAD-visual-only, 5xFAD-combined. 3. Stimulation: 1 h/day, 5 days/week, 4 weeks (20 sessions). 4. Weekly: open field, NOR. 5. Post-treatment: - Ex vivo LFP power spectral analysis (gamma 30-80 Hz) - ELISA: soluble Abeta40, Abeta42 (hippocampus) - IHC: Iba1 microglia morphology, 6E10 plaque burden, AQP4 (glymphatic) 6. Primary: soluble Abeta42 ELISA; secondary: LFP gamma power, NOR, plaque count.
LINKED HYPOTHESES
Source: task:a989715e-c687-4558-91ca-74fce1474bd2
🧫 Experiment Extras
ESTIMATED COST
$72,000
TIMELINE
14 months
PATHWAY
40 Hz oscillation -> glymphatic activation -> microglial Abeta clearance
MARKET PRICE
$0.50
STATUS
proposed
Scoring Dimensions
Prediction Markets (1 direct, 0 via hypothesis — 1 total)
Gamma Oscillation Enhancement SynergyYES 68% · Liq $100 · active▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.