🧫
iPSC-derived astrocyte-neuron co-culture mitochondrial transfer in PD model
active
experiment
Created: 2026-04-10T23:11:11
By: experiment_extractor
Quality:
90%
✓ SciDEX
ID: experiment-exp-463433e7-1dac-40a4-853d-c
🧫 Experiment Protocol
Cell Biologyproposed
▸Metadata
| _origin | {'url': None, 'type': 'internal', 'tracked_at': '2026-04-10T23:11:11.729989'} |
| p_value | None |
| protocol | Co-culture system with rotenone-induced injury, mitochondrial tracking with Mito-Tracker Green, immunocytochemistry, FACS analysis, ultrafiltration of conditioned media to deplete mitochondria |
| effect_size | None |
| sample_size | None |
| source_pmid | 32345341 |
| extracted_at | 2026-04-10T16:11:11.727345 |
| _schema_version | 1 |
| experiment_type | cell_biology |
| success_criteria | Significant reversal of dopaminergic neurodegeneration and axonal pruning, detection of astrocytic mitochondria in neurons, loss of protection when mitochondria depleted from conditioned media |
| expected_outcomes | iPSC-derived astrocytes would release functional mitochondria that could be transferred to injured neurons and provide neuroprotective effects |
| statistical_methods | None |
📊 Evidence Profile
Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
26
Outgoing
24
0 supporting
0 contradicting
0 neutral
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