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Experiment Proposal: h-var-e95d2d1d86
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experiment proposal
Created: 2026-04-28T12:58:59
By: forge_experiment_proposal_generator
Quality:
75%
✓ SciDEX
ID: experiment_proposal-59785671-1a01-4297-a
🧬 Experiment Proposal
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▸Metadata
| citations | ['31076275', '35151204', '36450248', '37384704', '38642614', '39964974', '27929004', '31578527', '36211804', '33127896', '28714589', '30936556', '34982715'] |
| generated_at | 2026-04-28T19:58:59.205388+00:00 |
| intervention | Stereotaxic injection of AAV9-CaMKIIα-DIO-ChR2-eYFP (Addgene #27056) into bilateral CA1 stratum pyramidale (AP -2.0, ML ±1.5, DV -1.4 mm from bregma) in PV-Cre mice (Jax #008069); control groups recei |
| key_controls | ['AAV9-CaMKIIα-DIO-eYFP injected PV-Cre mice ( specificity control for viral transduction without channelrhodopsin)', 'APP/PS1 mice with PBS injection ( vehicle control for surgical procedure)', 'Non- |
| model_system | APP/PS1dE9 mice (Jax #005864) on C57BL/6J background; both sexes; experiment onset at 3 months of age (pre-plaque stage based on PMID 31076275); Cre-negative littermates serve as controls for AAV-PV-C |
| timeline_weeks | 14 |
| _schema_version | 1 |
| null_hypothesis | Closed-loop optogenetic activation of PV interneurons in APP/PS1 mice does not restore theta-gamma coupling, prevent amyloid-induced synaptic dysfunction, or improve spatial memory beyond the level ac |
| primary_readout | Theta-gamma coupling modulation index calculated as the phase-amplitude coupling metric from Iac选 et al. (2009) Nature Methods, computed from 30 minutes of LFP recorded during exploration of novel spa |
| estimated_cost_usd | 287500.0 |
| secondary_readouts | ["Theta frequency power (6-10 Hz) and gamma frequency power (25-100 Hz) spectral density via Welch's method", 'CA1 PV interneuron firing rate and action potential waveform parameters from juxtacellula |
| expected_effect_size | Primary outcome (theta-gamma modulation index): Cohen's d = 0.90 (0.90) between APP/PS1-PV-ChR2 closed-loop and APP/PS1-sham groups, representing restoration to 80% of wild-type values. This effect si |
| statistical_approach | Linear mixed-effects model (LMM) for primary readout with fixed effects of genotype (APP/PS1 vs WT), stimulation condition (closed-loop ChR2, continuous, sham), timepoint (2, 4, 6 weeks, post-stimulat |
| sample_size_rationale | Power analysis performed in G*Power 3.1 for mixed ANOVA (within-between interaction). Assumptions: medium-to-large effect size (f = 0.35, derived from literature), α = 0.05, power = 0.85, 4 groups, 6 |
| falsification_criterion | The hypothesis is falsified if: (1) Theta-gamma coupling modulation index in APP/PS1-PV-ChR2 closed-loop group fails to exceed sham controls by ≥0.10 at 6 weeks (primary endpoint), even if wild-type c |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
1
0 supporting
0 contradicting
0 neutral
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