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Experiment Proposal: h-bdbd2120
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experiment proposal
Created: 2026-04-28T13:00:29
By: forge_experiment_proposal_generator
Quality:
75%
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ID: experiment_proposal-afa252d5-e3ae-4839-9
🧬 Experiment Proposal
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▸Metadata
| citations | ['31076275', '35151204', '36450248', '27929004', '31578527', '36879142', '37384704', '38642614', '39964974', '33127896'] |
| generated_at | 2026-04-28T20:00:29.804839+00:00 |
| intervention | 40 Hz audiovisual sensory entrainment delivered via custom Arduino-controlled device: 40 Hz square-wave LED array (luminance 250 cd/m², 50% duty cycle) + 40 Hz click train (2 ms duration, 70 dB SPL) c |
| key_controls | ['APP/PS1ΔE9 sham-stimulated mice (0.5 Hz stimulation, no entrainment) — disease model negative control', 'C57BL/6J wild-type mice with 40 Hz stimulation — positive control for entrainment efficacy', |
| model_system | APP/PS1ΔE9 mice on C57BL/6J background, both sexes, 6 months old at intervention start (moderate Aβ pathology burden), n=30 per group (power 0.80, α=0.05, two-tailed, expected d=0.85 for primary outco |
| timeline_weeks | 18 |
| _schema_version | 1 |
| null_hypothesis | 40 Hz audiovisual gamma entrainment (1 hour/day for 6 weeks) does not restore hippocampal-prefrontal cortical synchrony, does not reduce Aβ or tau pathology, does not activate microglial phagocytosis, |
| primary_readout | Hippocampal-prefrontal cortical gamma coherence measured via bilateral silicon probe LFP recordings (32 channels) during active stimulation and 30-min post-stimulation; primary endpoint is phase-locki |
| estimated_cost_usd | 287500.0 |
| secondary_readouts | ['Aβ40/Aβ42 levels in hippocampus and prefrontal cortex by ELISA (invitrogen)', 'Aβ plaque density by Thioflavin-S immunohistochemistry (stereology-based plaque count in visual cortex, hippocampus, PF |
| expected_effect_size | Cohen's d = 0.85 for hippocampal-cortical gamma coherence (primary), d = 1.0 for Aβ plaque reduction (literature-based, Martorell et al. 2019), d = 0.7 for cognitive improvement (NOR discrimination); |
| statistical_approach | Mixed-effects two-way ANOVA (treatment × time) for longitudinal readouts; two-tailed Welch's t-test for post-hoc pairwise comparisons with Bonferroni-Dunn correction for 8 secondary outcomes (adjusted |
| sample_size_rationale | Power calculation (G*Power 3.1): for two-group comparison with d=0.85, α=0.05, power=0.80, n=24/group minimum; inflated to n=30/group to accommodate mixed-effects model df loss and potential cage outl |
| falsification_criterion | Experiment falsifies hypothesis if: (1) hippocampal-prefrontal gamma PLV does not significantly differ between 40 Hz and sham groups (p > 0.00625 after multiplicity correction), OR (2) Aβ plaque burde |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
1
0 supporting
0 contradicting
0 neutral
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