Figure 6: HCAR2 inhibits pyroptosis and tight junction damage in mMECs induced by S. aure...

HCAR2 inhibits pyroptosis and tight junction damage in mMECs induced by S. aureus through downregulation of CMPK2 expression. a) Transmission electron microscopy was used to observe the mitochondrial morphology in mMECs, with red arrows indicating mitochondria and a scale bar of 2 µm. b) The mitochondrial membrane potential of mMECs was assessed using the JC‐1 membrane potential assay kit, with a scale bar of 100 µm. c) dsDNA antibodies and Mitotracker were used to label dsDNA and mitochondria, followed by imaging with a laser confocal microscope. Red arrows indicate co‐localization of dsDNA and mitochondria, with a scale bar of 2 µm. d, e) qPCR was performed to detect specific primers for mtDNA (D‐loop, ND2) and nDNA (Tert), and to determine the relative content of mtDNA. f) qRT‐PCR was used to measure mRNA levels of CMPK2 in mMECs. g) Western blot analysis was employed to detect CMPK2 protein expression in mMECs. h) TUNEL staining of mMECs, with red fluorescence representing TUNEL‐

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